DIG RNA标记试剂盒DIGRNALabelingKit(SP6/T7)：RNAlabelingwithdigoxigenin-UTPbyinvitrotranscriptionwithSP6andT7RNApolymeraseRNA是由SP6或T7RNA聚合酶催化的体外转录反应里被标记的。在转录反应过程中，核苷酸类似物Digoxigenin-11-UTP(DIG-UTP)是被利用来替代UTP(或其它***性标记了的核苷酸)的角色。在新合成的RNA中，约每25个碱基便掺入了DIG-UTP。可标记转录反应2×10标记反应后，DIG-标记RNA可用于各种杂交技术，包括：●Northern印迹●Southern印迹●Dot/Slot印迹●菌落筛选●噬菌斑杂交●原位杂交产品号产品规格厂商价格117502511175025910Kit：(2x10labelingreactions)Roche6468DIGRNALabelingKit(SP6/T7)ApplicationRNAlabelingwithDIG-11-UTPbyinvitrotranscriptionwithSP6andT7RNAPolymerases.DIG-labeled“runoff”transcriptsaresynthesizedwithhighefficiencyandcanbeusedinnorthernblots,Southernblots,insituhybridization,plaqueorcolonylifts,andRNaseprotectionexperiments.Contents1.pSPT18DNA,40l,0.25mg/ml2.pSPT19DNA,40l,0.25mg/ml3.ControlDNA1,pSPT18-Neo,20l,0.25mg/mlcleavedwithPvuII4.ControlDNA2,pSPT19-Neo,20l,0.25mg/mlcleavedwithPvuII5.DIG-labeledControlRNA,100l,100ng/lDIG-labeled“antisense”neoRNAdimethyldicarbonatetreateddouble-distilledwater6.UnlabeledControlRNA,20l,200g/mlneopoly(A)“sense”RNAindimethyldicarbonatetreateddouble-distilledwater7.NTPLabelingMixture,40l,10xconc.10mMATP,CTP,GTP,6.5mMUTP,3.5mMDIG-11-UTP,pH7.58.TranscriptionBuffer,40l,10xconc.9.DNaseI,RNase-free,40l,10U/l10.RNaseInhibitor,20l,20U/l11.SP6RNAPolymerase,20l,20U/l12.T7RNAPolymerase,20l,20U/lQualityTestprinciple:TheDNAtemplatetobetranscribedisclonedintothepolylinkersiteofappropriatetranscriptionvectors,whichcontainpromotersforSP6and/orT7RNAPolymerases.Afterlinearizationatasuitablesite,RNAistranscribedinthepresenceofDIG-UTP.Understandardconditions,approximately10goffull-lengthDIG-labeledRNAistranscribedfrom1gtemplate.