上海启因生物科技有限公司

内参基因引物
HUMAN:
hGAPDH ((Glyceraldehyde‐3‐phosphatedehydrogenase ‐ NM_002046):
Forward5’‐CAATGACCCCTTCATTGACC‐3’  
Reverse5’‐GACAAGCTTCCCGTTCTCAG‐3’  
hG6PD(Glucose‐6‐phosphatedehydrogenate ‐ NM_000402.3):
Forward5’‐GAGGCCGTGTACACCAAGAT‐3’  
Reverse5’ ‐TCAGGGAGCTTCACGTTCTT‐3’  
hHMBS(Hydroxymethylbilanesynthase ‐ BC019323.1):
Forward5’‐TGCAACGGCGGAAGAAAA‐3’
Reverse5’‐ AGCTGGCTCTTGCGGGTAC‐3’  
hHPRT1(Hypoxanthineguaninephosphoribosyltransferase1 ‐ NM_000194):
Forward5’–CCTGGCGTCGTGATTAGTGAT‐3’
Reverse5’–AGACGTTCAGTCCTGTCCATAA ‐ 3’  
MOUSE:
mGAPDH(Glyceraldehyde‐3‐phosphatedehydrogenate ‐ NM008084.2):
Forward5’ ‐ AGGTCGGTGTGAACGGATTTG‐3’   
Reverse5’ ‐TGTAGACCATGTAGTTGAGGTCA‐3’  
mG6PD(Glucose‐6‐phosphatedehydrogenate ‐ NM008062):
Forward  5’–CACAGTGGACGACATCCGAAA–3’  
Reverse5’ ‐ AGCTACATAGGAATTACGGGCAA‐3’  
mHMBS(Hydroxymethylbilanesynthase ‐ NM013551.2):
Forward  5’‐AAGGGCTTTTCTGAGGCACC–3’   
Reverse  5’‐AGTTGCCCATCTTTCATCACTG ‐3’ 
mHPRT1(Hypoxanthineguaninephosphoribosyltransferase1)
Forward5’–TCAGTCAACGGGGGACATAAA–3’
Reverse5’–GGGGCTGTACTGCTTAACCAG–3
RAT:
rGAPDH(Glyceraldehyde‐3‐phosphatedehydrogenase–AF_106860):
Forward5’ ‐ GTGCAGTGCCAGCCTCGTC–3’
Reverse5’ ‐ GGCAGCACCAGTGGATGCAG–3’
rHPRT(Hypoxanthineguaninephosphoribosyltransferase1‐ XM_343829):
Forward5’ ‐ GCCGACCGGTTCTGTCAT–3’
Reverse5’‐ TCATAACCTGGTTCATCATCACTAATC–3’
rCycloB(PeptidylprolylisomeraseB(cyclophilinB) ‐ NM_022536):
Forward5’ ‐ GGGCTCCGTTGTCTTCCTTT–3’
Reverse5’ ‐ GACTTTAGGTCCCTTCTTCTTATCGTT–3’
rHMBS(Hydroxymethylbilanesynthase ‐ NM_013168):
Forward5’ ‐ GGCTCAGATAGCATGCAAGAGA–3’
Reverse5’‐ TGGACCATCTTCTTGCTGAACA–3’
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