磷酸钙法转染HEK293T细胞实验方法哪位了解啊?有木有生物论坛有这...相关交流-蚂蚁淘在线

磷酸钙法转染HEK293T细胞实验方法哪位了解啊?有木有生物论坛有这...

mayitao /发布时间:1631554202 /浏览量:77

货号：	xy-C634Ra01
供应商：	上海信裕
数量：	1000
英文名：	RecombinantMitochondrialTranslationalInitiationFactor2(MTIF2)
保存条件：	-20℃

线粒体翻译起始因子2(MTIF2)重组蛋白

RecombinantMitochondrialTranslationalInitiationFactor2(MTIF2)

FORINVITROANDRESEARCHUSEONLY,NOTFORUSEINCLINICALDIAGNOSTICPROCEDURES!

Organismspecies	Rattusnorvegicus(Rat)
ProductNo.	xyC634Ra01
Source	Prokaryoticexpression
Host	E.coli
Purity	>90%
UOM	50ug
PredictedMolecularMass	53.1kDa
Concentration	n/a
Applications	SDS-PAGE;WB;ELISA;IP.
EndotoxinLevel	<1.0EUper1µg(determinedbytheLALmethod)
Residues	Met1~Arg201withtwoN-terminalTags,His-tagandGST-tag

Formulation

Suppliedaslyophilizedformin20mMTris,150mMNaCl,pH8.0,containing1mMEDTA,1mMDTT,0.01%sarcosyl,5%trehalose,andpreservative.

线粒体翻译起始因子2(MTIF2)重组蛋白 SEQUENCES

Thesequenceofthetargetproteinislistedbelow.
MNQKLLKLEKLLRFHTICRQLHSLSQRRLLAWWKHGSAPASPVWADWLCARPWPTDVLTGSALHQHRLLVTKKEKRPPRSQLSPIKTKKEVEVWVGMTVEELARAMAKDTDCVYEALLNTAIDIDSLEANSHLDEVWIKEVIKKAGMKLKWSKLKLERIRENKDAVRRPGADPALLKPRSPVVTIMGHVDHGKTTLLDKLR

USAGE

ReconstituteinsterileddH2O.

线粒体翻译起始因子2(MTIF2)重组蛋白 STORAGEANDSTABILITY

Storage: Avoidrepeatedfreeze/thawcycles.Storeat2-8^oCforonemonth.Aliquotandstoreat-80^oCfor12months.
StabilityTest: Thethermalstabilityisdescribedbythelossrateofthetargetprotein.Thelossratewasdeterminedbyacceleratedthermaldegradationtest,thatis,incubatetheproteinat37^oCfor48h,andnoobviousdegradationandprecipitationwereobserved.(ReferringfromChinaBiologicalProductsStandard,whichwascalculatedbytheArrheniusequation.)Thelossofthisproteinislessthan5%withintheexpirationdateunderappropriatestoragecondition.

线粒体翻译起始因子2(MTIF2)重组蛋白 AbouttheMarker(complimentary)

EffectiveSizeRange: 10kDato70kDa.
Proteinbands: 10kDa,14kDa,18kDa,22kDa,26kDa,33kDa,44kDaand70kDa.
Doubleintensitybands: The26kDa,18kDa,10kDabandsareatdoubleintensitytomakelocationandsizeapproximationofproteinsofinterestquickandeasy.
Ready-to-use: Noneedtoheat,diluteoraddreducingagentsbeforeuse.

温馨提示：不可用于临床治疗。

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