1.Bringsectionstowater

2.StaininCelestineblue,3mins

3.Washinwater

4.StaininCarazziHaemalum3minutes5.Washinwater

6.BlueinScottstapwater

7.Washinwater

8.Rinsein95%alcohol

9.StaininPicro-orange2mins

10.Washinwateruntilonlyredbloodcellsareyellow

11.Staininacidfuchsin5mins

12.Washinwater

13.StaininequalpartsPicro-orangeand80%alcohol

14.DifferentiateinphosphomolyBDicacidfor8mins

15.Washinwater

16.Staininanalineblue,8mins

17.Washinwater

18.Dehydrate,clear.MountsectionsinDPX

---

RESULTS:

--Fibrin-Brightred

--Muscle-Red

--Collagen-Blue

--Redbloodcells-Yellow/Orange

---

SOLUTIONS:

1.0.2%orangeGinsaturatedalcoholicPicricAcid

2.1%Acidfuchsinin3%TrichloroaceticAcid

3.1%PhosphomolybdicAcid

4.2%AnalineblueW5in2%AceticAcid(solubleBlue)

NOTE:NoresponsibilityisassumedbyTheUniversityofNottinghamortheQueensMedicalCentreNHSTrustforanyinjuryand/ordamagetopersonsorpropertyasamatterofproductsliABIlity,negligenceorotherwise,orfromanyuseoroperationofanymethods,products,instructionsorideascontainedinthematerialherein.ItistheusersresponsibiltytoensurethatallproceduresarecarriedoutaccordingtoappropriateHealthandSafetyrequirements.

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