SECTIONS

4micronParaffinwaxsections

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SOLUTIONS

1. AlcianBluepH3.1
   • AlcianBlue1g
   • 0.5%AceticAcid100ml

2. AlcianBluepH2.5
   • AlcianBlue1g
   • 3%AceticAcid100ml

3. AlcianBluepH1.0
   • AlcianBlue1g
   • 0.1MHydrochloricAcid100ml

4. AlcianBluepH0.2
   • AlcianBlue1g
   • 10%SulphuricAcid100ml

5. 0.1%Nuclearfastred
   • Nuclearfastred0.1g
   • AluminiumSulphate2.5g
   • Distilledwater100ml

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METHOD

1. Takesectionstowater
2. StaininalcianbluesolutionpH2.5for5minutes
3. Washwellinwater
4. Counterstainin0.1%nuclearfastredfor5minutes
5. Washinwater
6. Dehydrate,clear.MountsectionsinDPX

RESULTS

• Mucins-BrightBlue
• Nuclei-Red

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NOTES

1. TofurtheridentifyspecifictypesofacidmucinsyouneedtousetheotherpHsolutions.
   • Stronglysulphatedmucins-usepH0.2AlcianBlue
   • Weaklysulphatedmucins-usepH2.5orpH1.0AlcianBlue

2. WhenusinglowpHsolutionsdrainandblotdryinsteadofwashingatstage(3)ofthemethod.

   NOTE:NoresponsibilityisassumedbyTheUniversityofNottinghamortheQueensMedicalCentreNHSTrustforanyinjuryand/ordamagetopersonsorpropertyasamatterofproductsliABIlity,negligenceorotherwise,orfromanyuseoroperationofanymethods,products,instructionsorideascontainedinthematerialherein.ItistheusersresponsibiltytoensurethatallproceduresarecarriedoutaccordingtoappropriateHealthandSafetyrequirements.

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