10XHC(7DropoutAminoAcidLiquid)

2.4gtyr3.2gile2.0gphe4.0gglu8.0gthr4.0gasp6.0gval16.0gser0.8garginfinalvolumeof4L-Autoclave

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10XYNB

58gYNB(w/oaminoacidsandw/oammoniumsulfate)200gammoniumsulfateinafinalvolumeof4L-Autoclave

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AminoAcidSolutions(notetheseareNOT10Xsolutions,seebelow)

URA1mg/mlADE1mg/mlLYS10mg/mlMET2mg/mlTRP10mg/mlLEU20mg/mlHIS10mg/ml

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ExampleRecipeofCompletePlates

20gofAgarin609mldistilledwaterina2LflaskAutoclave20minutesAddthefollowing:

100ml	20%sterileglucose
100ml	10XYNBsolution
100ml	10XHC-7aadropoutsolution
35ml	URAsolution
20ml	ADEsolution
12ml	LYSsolution
10ml	METsolution
8ml	TRPsolution
4ml	LEUsolution
2ml	HISsolution

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FOAplates

20gofAgarin609mldistilledwaterina2LflaskAutoclave20minutesAddthefollowing:

100ml	20%sterileglucose
100ml	10XYNBsolution
100ml	10XHC-7aadropoutsolution
35ml	URAsolution
20ml	ADEsolution
12ml	LYSsolution
10ml	METsolution
8ml	TRPsolution
4ml	LEUsolution
2ml	HISsolution

Letflaskcoolto50degC,thenadd1.0gof5-FOA.

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