StorageinStabAgar:MoststrainsofE.colicanbestoredforonetotwoyearsinstabagar.

1. Useairtight,autoclavedvialscontaining2/3full(appr.1ml)ofmedium.

2. Inoculateeachvialwithasinglecolony.

3. Leavethecapofthevialsightlylooseandincubateovernightat37C.

4. Sealthevialtightlyandstoredatroomtemperatureinadarkplace.

5. Toreviveastoredstrain,streakontoLBplatecontainingappropriateantibioticsforasinglecolony.

StorageinglycerolSolution:MoststrainsofbacteriaincludingE.coli,andfungi(sporesispreferred)canbestoredforonetotwoyearsinglycerolsolutionat-20C.At-70Ctheycanbestoredalmostforlifetime.

1. Prepare0.5mlofglycerolsolutionin2.0mlscrew-capvials,autoclave(15min,121C),cool,andstorethematroomtemperature.

2. Transferonedrop(0.5ml)ofanovernight-growncultureintoavial.

3. Voltex,storeat-20or-70C(-70Cstorageispreferredforlong-termmaintenance).

4. Toreviveastoredstrain,streakontoLBplatecontainingappropriateantibioticsforasinglecolony.

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