Calciumisessentialforalllivingorganisms,whereCa?2sequestrationandreleaseintoandoutofthecytoplasmfunctionsasasignalformanycellularprocesses.99%ofcalciumisfoundinbonesandteethwiththeremaining1%foundinthebloodandsofttissue.Serumcalciumlevelsaretightlycontrolled(8.4-11.4mg/dL)andanyvariationoutsidethisrangecanhaveseriouseffects.Calciumplaysaroleinmediatingtheconstrictionandrelaxationofbloodvessels,nerveimpulsetransmission,musclecontraction,andhormonesecretion.Calciumionchannelscontrolthemigrationofcalciumionsacrosscellmembranes,permittingtheactivationandinhibitionofawidevarietyofenzymes.Causesoflowcalciumlevelsincludechronickidneyfailure,vitaminDdeficiency,andlowbloodmagnesiumlevelsthatcanoccurinseverealcoholism.GeneTex’sColorimetricCalciumAssayKitutilizesthechromogeniccomplex(=575nm)formedbetweencalciumionsand0-cresolphthaleintoprovideasimpleassayinthephysiologicallyimportantrangeofcalciumconcentration0.4-100mg/dL(0.1-25mM).

‧Detectionmethod-Colorimetric(max=575nm)
‧Sampletype-CellandTissuelysates,culturemedia,urine,plasmaandserum,aswellasmanyotherbiologicalfluids
‧Speciesreactivity-Mammalian
‧Kitsize-250assays
‧Applications-Thekitcandetectcalciumconcentration0.4-100mg/dL(0.1-25mM).

FeaturesandBenefits
‧Simpleprocedure;takeslessthan20minutes
‧Fastandconvenient
‧Theassayissensitive,stableandhigh-throughputadaptable
‧CalciumAssayKitutilizesthechromogeniccomplexformedbetweencalciumionsand0-cresolphthaleintoprovideasimpleassay

KitContents:
CalciumAssayBuffer
ChromogenicReagent
CalciumStandard(500mM)

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