HistonePeptideH323:42K23me3_R26me2a_K27ac(H3.1t)

ProductCode:SP-His_0606

C-terminallybiotinylatedsyntheticHistonepeptidecarrying3modifications(K23me3,R26me2a,K27ac)derivedfromhumanHistoneH3,H3.1t(Swiss-ProtId(s):Q16695).
Aminoacidsequence:Ac-Lys(Me3)-VA-Arg(Me2a)-Lys(Ac)-SAPATGGVKKPHRYR-Lys(Biotinoyl)-NH2
Amount:1vialcontaining10nmol(appr.35µg)
Purity:>70%(HPLC-MS)
Deliveryformat:Freeze-driedinambertypeglassvials
Application(s):WesternBlot,Bindingexperiments,ELISA,Enzymekinetics,Pulldownassays,SubstrateSpecifity
Indication(s)/Topic(s):Cancer,Phosphatase,Kinase,Methyltransferase,Acetyltransferase,Histones,Phosphatase,Generegulation&expression,Epigenetics,Cellsignaling,Nucleosome,Aging
Deliverytime:2-5days

JPTsHistoneCodePeptides
Ashistonesareamajorfocusofepigenetics,JPTscomputationalscientistscompiledauniquelibraryofsyntheticposttranslationallymodifiedandunmodifiedhumanhistonepeptidesthatisnowavailableasindividualbiotinylatedpeptides.Thepeptidelibraryconsistsofmorethansevenhundred20merpeptidesfrom29histonefamilies(e.g.H2A[1,19,85...],H2B[1,22,42...],H3[1,23,43...],H4[1,14,44...]).PeptidescarryPTMsatreportedsites(e.g.acetylation,methylation,phosphorylation,citrullination)invariouscombinationsrepresentingnaturalvariants.
JPTshighlyskilledandcommittedscientificstaffensuresthatthemostappropriatemethodsandtechniquesareselectedforeverysynthesisproject.AllofJPTscatalogpeptidesareprovidedwithHPLC-MSanalysestoconfirmtheidentityanddemonstratethehighqualityofourpeptides.

BenefitsofJPTsHistoneCodePeptides
-WholeproductionperformedinaregulatedEuropeanenvironmentaccordingtoDINENISO9001:2015&GCLPstandards;auditswelcome!
-Forhigh-throughputscreeningthewholeHistoneCodePeptidelibraryisalsoavailabledisplayedonpeptidemicroarrays
-Provisionoffreezedriedaliquotsforenhancedstability
-Proventrackrecordformanyapplications

HistonePeptideH323:42K23me3_R26me2a_K27ac(H3.1t)

SelectedReferencesforHistonePeptideTools
"HistoneH2AandH4N-TerminalTailsarePositionedbytheMEP50WD-RepeatProteinforEfficientMethylationbythePRMT5ArginineMethyltransferase"
Burgosetal.,J.Biol.Chem.(2015)-PMID:25713080
"H3K23me2isaNewHeterochromaticMarkinCaenorhabditiselegans"
Vandammeetal., NucleicAcidsRes.(2015)-PMID:26476455
"StructureoftheArginineMethyltransferasePRMT5-MEP50RevealsaMechanismforSubstrateSpecificity"
Hoetal.PLOSONE(2013)-PMID:23451136
"UseofHigh-DensityHistonePeptideArraysforParsingtheSpecificityofaHistone-ModifyingEnzymeComplex"
Shechteretal,ApplicationNote(2013)(fulltext)
"ComprehensivePeptideMicroarraysforHistoneResearch"
Schnatbaumetal,32thEPS(2012)(fulltext)

ApplicationNotes
"UseofHigh-DensityHistonePeptideArraysforParsingtheSpecificityofaHistone-ModifyingEnzymeComplex"
Shechteretal.ApplicationNote(2013)(fulltext)
"ComprehensiveCharacterizationofAntibodiesDirectedtowardsEpigeneticHistone-Modifications"
Maschetal.ApplicationNote(2015)(fulltext)

TestimonialforHistoneCodePeptideMicroarray
"JPTsnewextremelyhigh-densityhistonepeptidearrayisadramaticleapforwardforanalysisofthehistonecodehypothesis.Thecompletesequencecoverageacrosscorehistonesandhistonevariantsaswellasthepresenceofcommonandrarehistonepost-translationalmodifications "aloneandincombination"makesthisanunparalleledtoolforstudyingthe"writers"and"readers"ofthehistonecode.MylaboratoryhasusedthesearraystoaskthesequenceandPTMdependenceofahistonewriterenzymeandwehavediscoveredanactivityspecifyingcodethatwillkeepusbusyforyearstocome."
DavidShechter,PhD,DepartmentofBiochemistry,AlbertEinsteinCollegeofMedicine,YU(NY,USA)