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DescriptionThemouseTransforminggrowthfactorbeta-1(TGFb1)KitisasandwichELISA.ThecaptureantibodyisamonoclonalTGFb1antibodypre-coatedontothe96-wellstripplatesprovidedinthekit.MousetestsamplesandstandardsofknownTGFb1concentrationareaddedtothesewellsandallowedtocomplexwiththeboundTGFb1antibody.AbiotinylatedTGFb1polyclonalantibodyisthenadded.Thisdetectionantibodybindstotheantigenthuscompletingthesandwich.Afterwashing,anenzymeAvidin-Biotin-Peroxidasecomplex(ABC)isaddedwhichbindstothesecondantibody.TheperoxidasesubstrateTMBisaddedtoinduceacolouredreactionproduct.TheintensityofthiscolouredproductisdirectlyproportionaltotheconcentrationofTGFb1presentinthesamples.Thepurposeofthiskitisthein-vitroquantitativedeterminationofmouseTGFb1cellculturesupernatants,serum,plasma(EDTA)andurine.Acidactivationisrequiredforbestactivity.Checkthatsamplesareneutralizedpriortorunningtheassay.FreeTGFB1ishighlylabile.Thiskithasbeenconfiguredforresearchuseonlyandisnottobeusedindiagnosticorclinicalprocedures.
BatchNo.Seeproductlabels
OtherNamesTGFB1;TGFB;TGF-beta-1;
AccessionP04202TGFB1_MOUSE;
SpecificityHuman;Mouse;Rat;
Cross-reactivityThecrossreactivitywithTGFβ2,TGFβ3andTGFβ5is< 1%.
StorageStoreat4øC
KitcomponentsTheELISAkitboxcontains1x96pre-coatedstripplates,proteinstandards,detectionreagents,substratebufferandpreciseinstructions.
Range15.6pg/ml-1,000pg/ml
Sensitivity< 2 pg/ml
KitprotocolClick to download the kit protocolBEK-2095-1P_as_at_Jun2017_protocol.pdf