GeneralInformation

Product

CD4Antibody

Description

AffinityPurifiedT-cellsurfaceglycoproteinCD4 AntibodyC-epitope

VerifiedApplications

ELISA,IHC,IP,WB

Host

Rabbit

SpeciesCrossReactivity

Human,Monkey,Mouse,Rat

Immunogen

SyntheticpeptidecorrespondingtoHumanCD4aa437-458(Cterminal).Theselectedpeptidewaspost-syntheticallymodifiedtoachievehighestantigenicitybeforecouplingtocarrierproteinusingheterobifunctionalcrosslinkerforImmunogenpreparation.

Sequence:

RLLSEKKTCQCPHRFQKTCSPI

Accession#

NCBI: NP_000607.1

PhysicalProperties

Quantity

100µg

Volume

200µl

Concentration

0.55-0.75µg/µlinantibodystabilizationbuffer

Form

AffinityPurified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰Cforlongtermstorage

ApplicationProtocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

WesternBlot

1:500.PredictedMolecularweight:51kDa

CD4-112APlabelsabandof28kDa,theidentityofthisbandisnotfullyunderstood,butmayrepresentaproteolyticdegradationproductofCD4.

Protein

Uniprot#

P01730

Overview

AccesoryproteinforMHCclass-IIantigen/T-cellreceptorinteraction.

MolecularFunction

AdaptiveImmunity;Host-virusinteraction;Immunity

SubcellularLocation

Cellmembrane;Single-passtypeImembraneprotein

Structure

Contains3Ig-likeC2-typedomains;Contains1Ig-likeV-typedomain

AlternativeNomenclature

CD4antibody

CD4(L3T4)antibody

CD4antigen(p55)antibody

CD4antigenantibody

CD4moleculeantibody

CD4receptorantibody

CD4+Lymphocytedeficiency,includedantibody

CD4mutantibody

L3T4antibody

Leu3antibody

Ly-4antibody

LymphocyteantigenCD4antibody

p55antibody

TcelldifferentiationantigenL3T4antibody

TcellOKT4deficiency,includedantibody

T-cellsurfaceantigenT4/Leu-3antibody

T-cellsurfaceglycoproteinCD4antibody

W3/25antigenantibody