NewEastBiosciences主营：专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
华东地区代理NewEastBio专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。

ProductHighlightConfiguration-specificmonoclonalantibodythatrecognizesGi-GTP,butnotGi-GDPTheonlyavailableGiactivationassaykitdirectlymeasuresthelevelofGi-GTPProvidingreliableresultswithconsistentreproducibilitythanotherGiactivationassaykitsbasedoncertaindownstreamsignalingpathwaysProvidingshorterexperimentaltimeCouldbeusedtomonitortheactivationofGiincellsandintissuesbyimmunohistochemistryProductDescription

Astructurallydiverserepertoireofligands,fromphotonstolargepeptides,activatesGprotein-coupledreceptors(GPCRs)toelicittheirphysiologicalfunctions.Ligand-boundGPCRs,inturn,functionasguaninenucleotideexchangefactorscatalyzingtheexchangeofGDPboundontheGsubunitwithGTPinthepresenceofG,causingthedissociationoftheGsubunitfromtheGdimertoformtwofunctionalunits(GandG).BothGandGsubunitssignaltovariouscellularsignalingpathways.Basedonthesequenceandfunctionalhomologies,Gproteinsaregroupedintofourfamilies:Gs,Gi,Gq,andG12.

GifamilyisthelargestfamilyofGproteins.TheyrelaysignalsfrommanyGPCRstoregualtevariouSBIOLOGicalfunctions.TherewerenodirectmethodstomeasuretheactivationofGiproteinsbyreceptors(untilthisassaykit).Mostreportsusedoneofthedownstreampathway,i.e.theinhibitionofadenylylcyclases,asareadout.Alternatively,sensitivitytopertussistoxin(PTX)wasusedasanindicatorofpossIBLeGiproteinsinvovledinasignalingpathway.

NewEastBiosciencesGiActivationAssayKitprovidesADIrectmeasurementoftheactivationofGiproteins.ThisisasimpleandfasttooltomonitortheactivationofGi.Eachkitprovidessufficientquantitiestoperform20assays.

NewEastBiosciencesGiActivationAssayKitisbasedonthemonoclonalantibodyspecificallyrecognizingtheactiveGTP-boundGiproteins.ThismonoclonalantibodyhasmuchloweraffinitytowardstheinactiveGiproteins.Therefore,afteractivationbyreceptorsignals,activeGTP-boundGiproteinscouldbeimmunoprecipitatedbythismonoclonalantibodyandfurtherquantifiedbywesternblotwithanotheranti-Giantibody.

AssayPrinciple

NewEastBiosciencesGiActivationAssayKitisanimmunoprecipitation/westernblotassaytomeasurethelevelsofactiveGTP-boundGiproteins,eitherfromcellextractsorfrominvitroGTPSloadedGiproteins.Briefly,theanti-activeGimonoclonalantibodywillspecificallybindtoactiveGiprotein.Thisantibody/GicomplexwillthenbepulleddownbyproteinA/Gagarose.TheprecipitatedactiveGiproteinswillbedetectedbyimmunoblotswithanotheranti-Giantibody.

ProductManualRelatedProductsCatalog#NameSizePriceDataSheetiActivationAssayKit">80301GiActivationAssayKit20assays$637i-GTP">26901ActiveGi-GTPMonoclonalAntibody30L$495iMouseMonoclonalAntibody">26003Anti-GiMouseMonoclonalAntibody100L$275iRabbitPolyclonalAntibody">21006Anti-GiRabbitPolyclonalAntibody100L$275Publications:1.IdentificationofnovelsignallingrolesandtargetsforGalphaandGbetagammadownstreamoftheinsulin-likegrowthfactor1receptorinvascularsmoothmusclecellsBiochemJ.2013Feb15;450(1):209-192.CRHactivationofdifferentsignalingpathwaysresultsindifferentialcalciumsignalinginhumanpregnantmyometriumbeforeandduringlaborJClinEndocrinolMetab.2012Oct;97(10):E1851-613.CXCR4activationdefinesanewsubgroupofSonichedgehog-drivenmedulloblastomaCancerRes.2012Jan1;72(1):122-324.RegulationofestrADIolandprogesteroneproductionbyCRH-R1and-R2isthroughdivergentsignalingpathwaysinculturedhumanplacentaltrophoblastsEndocrinology.2012Oct;153(10):4918-285.WNT-5AstimulatestheGDP/GTPexchangeatpertussistoxin-sensitiveheterotrimericGproteinsCellSignal.2011Mar;23(3):550-46.HeterotrimericGalpha(i)proteinsareregulatedbylipopolysaccharideandareanti-inflammatoryinendotoxemiaandpolymicrobialsepsisBiochimBiophysActa.2011Mar;1813(3):466-727.Estrogen-andxenoestrogen-inducedERKsignalinginpituitarytumorcellsinvolvesestrogenreceptor-interactionswithGprotein-iandcaveoliNISTeroidsVolume77,Issue5,April2012,Pages424–4328.Na/HexchangerregulatoryfactorscontrolparathyroidhormonereceptorsignalingbyfacilitatingdifferentialactivationofG(alpha)proteinsubunitsJBiolChem.2010Aug27;285(35):26976-869.Beta-arrestin-butnotGprotein-mediatedsignalingbythe"decoy"receptorCXCR7ProcNatlAcadSciUSA.2010Jan12;107(2):628-3210.StructuralbasisforactivationoftrimericGiproteinsbymultiplegrowthfactorreceptorsviaGIV/GirdinMolBiolCell.2014Nov5;25(22):3654-7111.CanonicalandNon-canonicalG-proteinSignalingHelpsCoordinateActinDynamiCSToPromoteMacrophagePhagocytosisofZymosanMolCellBiol.2014Nov15;34(22):4186-9912.FattyAcid-bindingProtein5(FABP5)RegulatesCognitiveFunctionBothbyDecreasingAnandamideLevelsandbyActivatingtheNuclearReceptorPeroxisomeProliferator-activatedReceptor/(PPAR/)intheBrainJBiolChem.2014May2;289(18):12748-58

华东地区代理NewEastBio专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。