Rabbit MonoclonalRevmab/Anti-Histone H3 K36M Rabbit Monoclonal Antibody, Clone RM193; Histone H3, K36M Mutant (50ug)/31-1085-00/1 Ea
RevMAb Biosciences USA, Inc has developed a revolutionary monoclonal antibody generation method which allows development of monoclonal antibodies without generation of hybridoma. The recombinant antibodies are developed from a broad range of antigen-...
ProductDetailsProductNameAnti-HistoneH3K36MRabbitMonoclonalAntibody[RM193]ProductDescriptionRabbitmonoclonaltoHistoneH3K36M;HistoneH3,K36MMutantCatalogNo.31-1085-00CloneNameRM193ProductDataSheetAnti-HistoneH3K36MRabbitMonoclonalAntibodyDataSheetRM193SpecificityRM193reactstotheHistoneH3K36Mmutant.NocrossreactivitywithwildtypeHistoneH3.ImmunogenApeptidecorrespondingtoHistoneH3K36MmutantApplicationWesternBlot
ELISA
Immunohistochemistry
Immunocytochemistry
ChromatinIP
SpeciesReactivityAllConjugateNoneIntendedUseResearchUseOnly.PropertiesFormLiquidStorageInstructionsStoreat-20.0°CStABIlityStablefor1Yearat-20.0°CfromdateofreceiptStorageBuffer50%Glycerol/PBSwith1%BSAand0.09%sodiumazideVolume/Size50ugConcentration1.0mg/mLPurityProteinAaffinitypurifiedfromananimalorigin–freeculturesupernatantCloneTypeMonoclonalIsotypeRabbitIgGUsageWesternBlot:0.5ug/mL–2ug/mL;
ELISA:0.5ug/mL-2ug/mL;
Immunohistochemistry(IHC):0.5–5ug/mL;
Immunocytochemistry(ICC/IF):0.5–5ug/mL.
KeywordsH3F3A;H3F3B;K36M;bonetumour;chondroblastomaReferencesforHistoneH3K36MMutantantibody[RM193]AmaryMFetal.TheH3F3K36MmutantantibodyisasensitiveandspecificMarkerforthediagnosisofchondroblastoma.Histopathology10.1111/his.12945(2016).IHC-P;Human.Readmore(PubMed:26844533)

D.Fangetal.ThehistoneH3.3K36MmutationreprogramstheepigenomeofchondroblastomasScience10.1126/science.aae0065(2016).ChIP;Human.Readmore(PubMed:27229140).ProductImages

WesternBlotanalysisofcelllysatespreparedfrom
293T transfected with a DNA
construct encoding
wild type or K36M mutant proteins of Histone H3.1
and H3.3, using anti-Histone H3 K36M clone RM193.

Immunohistochemicalstainingofformalinfixedand
paraffin embedded 293T cells transfected with a DNA
construct encoding Histone H3 K36M, stained with
anti-Histone H3 K36M clone RM193.

Immunohistochemicalstainingofformalinfixed
paraffin embedded (FFPE) Chondroblastoma tumor
cell tissue sections.

ImagecourtesyofAdrienneFlanagan,Department
of Histopathology, RONH, Stanmore, Middlesex, UK

RevMAb Biosciences USA, Inc has developed a revolutionary monoclonal antibody generation method which allows development of monoclonal antibodies without generation of hybridoma. The recombinant antibodies are developed from a broad range of antigen-specific B cells that are inaccessible to traditional hybridoma technology. This revolutionary method has successfully been applied to develop high quality recombinant rabbit and goat monoclonal antibodies.