exosomes外泌体实验方案.docx_相关交流-蚂蚁淘在线

exosomes外泌体实验方案.docx_

蚂蚁淘在线 /发布时间:1632331802 /浏览量:96

Immunoplates for Overall Exosome capture from human saliva.

Product	Black 1 standard 96-well format pre-coated plate
Sample type	Human saliva
Exosome capiture	Overall exosome
Starting material	Up to 100 µL of biological sample can be loaded per well. Unfractionated human saliva sample can be directly used for capture.
Product overview	Our multiwell plates are standard 96-well format where assays can be conducted as singletons and/or multiple wells. This enables optimization of a wide range of sandwich ELISA assays or other downstream applications, such as RNA extraction and proteomic analyses. Transparent, white and black plates are available depending on the downstream detection approach (colorimetric, luminometric and fluorimetric).
Antibody used for coating	Polyclonal rabbit antibodies
Coating	Covalent
Packaging	The plate is packaged in an opaque aluminum pouch which complies to food and pharmaceutical regulations. Easy to open and re-sealable by zip-lock feature.
Shipment and storage	Immunoplates are shipped at 4°C and storage conditions as recommended in the product insert.
Expiration date	Unopened: 2 year stored at 4°C Opened: 6 month stored at 4°C

Applications:

Proteomic analyses

Exosomes incorporate a wide range of membranes and cytosolic proteins involved in many cellular functions and reflecting both their endosomal origin and a protein fingerprint unique for parent cell type and condition. HBM Immunoplates are an useful platform for Exosome capture from different biological sources and allow quantification through ELISA technique of protein exosomal markers.

Work with exosomal RNA

Exosomes shuttle RNA molecules (mRNAs and miRNAs), both classes proved to be functional in target cell, being translated or modulating gene silencing, respectively. Overall, exosome molecular composition distinguishes resting from activated, healthy from diseased cells.

RNA extraction could be performed after capturing exosomes on the plate. Recommended for RNA extraction: HansaBioMed Exosome Total RNA Extraction Kit.

Total exosome quantification

Immunoplates are part of the Ready to Use Kit for capture and quantification. The kit contains all the necessary components exosome quantification.

Related products:

Exosome standards

RNA extraction kits

Quantification kits

================ 蚂蚁淘在线 ================

免责声明：本文仅代表作者个人观点，与本网无关。其创作性以及文中陈述文字和内容未经本站证实，对本文以及其中全部或者部分内容、文字的真实性、完整性、及时性本站不做任何保证或承诺，请读者仅作参考，并请自行核实相关内容