Rabbit MonoclonalRevmab/Anti-Trimethyl-Histone H4 (Lys20) Rabbit Monoclonal Antibody, Clone RM208; H4K20me3, Histone H4 (Trimethyl Lys20)/31-1
RevMAb Biosciences USA, Inc has developed a revolutionary monoclonal antibody generation method which allows development of monoclonal antibodies without generation of hybridoma. The recombinant antibodies are developed from a broad range of antigen-...
ProductDetailsProductNameAnti-Trimethyl-HistoneH4(Lys20)RabbitMonoclonalAntibody[RM208]ProductDescriptionRabbitmonoclonaltoTrimethylatedHistoneH4Lysine20,H4K20me3,HistoneH4(TrimethylK20)CatalogNo.31-1096-00CloneNameRM208ProductDataSheetAnti-Trimethyl-HistoneH4(Lys20)RabbitMonoclonalAntibodyDataSheetRM208SpecificityRM208reactstoHistoneH4trimethylatedatLysine20(K20me3).ItmayslightlycrossreacttodimethylatedLysine20(K20me2)atahigherconcentration.Nocrossreactivitywithnon-modifiedLysine20,monomethylatedLysine20(K20me1),orothermethylationsinhistoneH4.ImmunogenAtrimethyl-peptidecorrespondingtotrimethyl-HistoneH4(Lys20).ApplicationWesternBlot(WB)
ELISA
MultiplexSpeciesReactivityHuman
VertebratesConjugateNoneIntendedUseResearchUseOnly.PropertiesFormLiquidStorageInstructionsStoreat-20.0°CStABIlityStablefor1Yearat-20.0°CfromdateofreceiptStorageBuffer50%Glycerol/PBSwith1%BSAand0.09%sodiumazideVolume/Size100ugConcentration1.0mg/mLPurityProteinAaffinitypurifiedfromananimalorigin–freeculturesupernatantCloneTypeMonoclonalIsotypeRabbitIgGUsageWesternBlot:1ug/mL–2ug/mL;
ELISA:0.5ug/mL-1ug/mL;
Multiplex:0.1ug/mL–0.5ug/mL.

Keywords.ProductImages

RM208specificallyreactstoHistoneH4trimethylatedat
Lysine 20 (K20me3). May slightly cross react to dimethylated
Lysine 20 (K20me2) at higher concentrations. No cross
reactivity with non-modified Lysine 20, monomethylated
Lysine 20 (K20me1), or other methylations in histone H4.

WesternBlotof(1)acidextractsofHeLacells;(2)
recombinant Histone H4. Using RM208 at 1 ug/mL,
showed a band of Histone H4 trimethylated at Lysine
20 in HeLa cells.

RevMAb Biosciences USA, Inc has developed a revolutionary monoclonal antibody generation method which allows development of monoclonal antibodies without generation of hybridoma. The recombinant antibodies are developed from a broad range of antigen-specific B cells that are inaccessible to traditional hybridoma technology. This revolutionary method has successfully been applied to develop high quality recombinant rabbit and goat monoclonal antibodies.