ProductSpecification:

AlternativeName:	TBP1,Tat-bindingprotein1,26Sproteaseregulatorysubunit6A,Proteasome26SsubunitATPase3
Clone:	TBP1-19
Host:	Mouse
Isotype:	IgG2b
Immunogen:	Recombinantfull-lengthhumanRpt5.
UniProtID:	P17980
Speciesreactivity:	Human,Mouse,Rat Rabbit
Specificity:	RecognizestheRpt5/S6asubunitofthe19Sregulatorysubunit
Applications:	IHC,WB
PurityDetail:	Partiallypurifiedascites.
Formulation:	Liquid.Contains10mMsodiumazide.
Shipping:	ShippedonBlueIce
LongTermStorage:	-20°C
ScientificBackground:	Theproteasomeiswidelyrecognisedasthecentralenzymeofnon-lysosomalproteindegradation.ItisresponsIBLeforintracellularproteinturnoveranditisalsocriticallyinvolvedinmanyregulatoryprocessesand,inhighereukaryotes,inantigenprocessing.The26Sproteasomeisthekeyenzymeoftheubiquitin/ATPdependentpathwayofproteindegradation.Thecatalyticcoreofthisunusuallylarge(2000kDa,450Åinlength)complexisformedbythe20Sproteasome,abarrelshapedstructureshownbyelectronmicroscopytocompriseoffourringseachcontainingsevensubunits.Basedonsequencesimilarity,allfourteen20Sproteasomalsubunitsequencesmaybeclassifiedintotwogroups,αandβ,eachgrouphavingdistinctstructuralandfunctionalroles.Theα-subunitscomprisetheouterringsandtheβ-subunitstheinnerringsofthe20Sproteasome.Observationsoftheeukaryoticproteasomeandanalysisofsubunitsequencesindicatethateachringcontainssevendifferentsubunits(α7β7β7α7)withamemberofeachsub-familyrepresentedineachparticle.Eachsubunitislocatedinauniquepositionwithintheα-orβ-rings.Inadditiontothe20Sparticle,the26Scomplexcontainsovertwentyadditionalproteins,ranginginmolecularweightfrom25to10kDa,locatedinadistinctcomplexcalledthe‘PA700proteasomeactivator’orthe‘19Scomplex’,andwhichdeterminessubstratespecificityandprovidesthemultipleenzymaticfunctionsnecessaryforproteolysisandviABIlity.Systematicanalysisofthesub-unitcomponentshaverevealedatleastsixmemberstobeATPasesbelongingtoanewfamilyofATP-bindingproteins,togetherwithafurtherfifteensubunitsthatlackthecapacitytobindATP,isopeptidasesandseveralotherproteinsthoughttoberesponsiblefortheunfoldingofaproteinsubstratepriortoinsertionintotheproteolyticcoreofthe20Sproteasome.
TechnicalInfo/ProductNotes:	ThehybridomasecretingtheantibodytosubunitRpt5/S6awasgeneratedbyfusionofsplenocytesfromBalb/cmicethathadreceivedrepeatedimmunisationwithafulllengthhumanrecombinantproteinwithSp2/0-Ag14myelomacells.Theantibody(cloneTBP1-19)hasbeencharacterisedbyone-dimensionalWesternblotting. Immunoblotting-SingledimensionSDS-PAGEofapurifiedrabbit26SproteasomepreparationfollowedbyWesternblottingatoptimaldilution(1:2500)givesamajorbandwitharelativemolecularweightofapproximately50kDa.Thereisaminorbandobservedrunningslightlyslowerthatmaybeduetomodification. Immunoprecipitation-Thisantibodyhasnotbeencharacterisedforthispurpose. Immunohistochemistry-Thisantibodyhasbeenshowntobesuitableforthispurposeinthesubcellularlocalisationofproteasomes.

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