Product Specification:
| Alternative Name: | Nitric oxide synthase (neuronal), NOS I |
| Host: | Rabbit |
| Immunogen: | Synthetic peptide corresponding to aa 1414-1434 (CRSESIAFIEESKKDTDEVFSS) of human brain NOS (nNOS; NOS1). |
| UniProt ID: | P29475 |
| Species reactivity: | Human, Mouse, RatChicken |
| Applications: | ELISA, ICC, IF, WB |
| Recommended Dilutions/Conditions: | Western Blot (1-5 µg/ml)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application. |
| Purity Detail: | Protein A-affinity purified. |
| Formulation: | Liquid. In PBS containing 0.05% sodium azide. |
| Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C. |
| Shipping: | Shipped on Blue Ice |
| Long Term Storage: | -20°C |
| Scientific Background: | The diffusible free radical gas nitric oxide (NO) affects a variety of physiological functions, and is a key regulator of the cardiovascular, nervous, and immune systems. NO is synthesized in many tissues from L-arginine, oxygen, and NADPH by three known isoforms of a heme-containing flavoprotein termed NO synthase (nNOS/NOS-I, iNOS/NOS-II, and eNOS/NOS-III). nNOS is a constitutively expressed neuronal NOS isoform that exits in its latent form until it is activated by the binding of calmodulin follo wing elevation of intracellular calcium levels. The C-terminus of nNOS contains a conserved serine residue, Ser1417, analogous to Ser1177 of the constitutively expressed endothelial NOS isoform (eNOS). Phosphorylation of Ser1417 is believed to regulate nNOS activation, particularly in glucose-sensing neurons, where inhibition of AMPK pathways by glucose and leptin serve to suppress nNOS activity, whereas activation of AMPK by insulin leads to nNOS activation. |
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