Addgene/pENN.AAV.CMVs.TurborRFP.WPRE.RBG/1unit/105548-AAV1

价格
¥7600.00
货号:105548-AAV1
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品牌:Addgene
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商品描述

Ordering

ItemCatalog #DescriptionQuantityPrice (USD)
Plasmid105548Standard format: Plasmid sent in bacteria as agar stab1$75
Add to Cart
AAV1105548-AAV1Virus (100 µL at titer ≥ 1×10¹3 vg/mL)and Plasmid.More Information
$380
Add to Cart
AAV5105548-AAV5Virus (100 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information
$380
Add to Cart
AAV8105548-AAV8Virus (100 µL at titer ≥ 1×10¹³ vg/mL)and Plasmid.More Information
$380
Add to Cart

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAAV
    (Search Vector Database)
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TurboRFP
  • PromoterCMV

Cloning Information

  • Cloning methodUnknown

Resource Information

  • Supplemental Documents
    • pENN.AAV.CMV.TurboRFP.WPRE.RBG (P2177).gb
  • Terms and Licenses
    • UBMTA
    • Evrogen Limited Use Label License for FPs
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Penn Vector Core number p2177

Information for AAV1 (Catalog # 105548-AAV1)(Back to top)

Purpose

Ready-to-use AAV1 particles produced from pENN.AAV.CMVs.TurboRFP.WPRE.RBG (#105548). In addition to the viral particles, you will also receive purified pENN.AAV.CMVs.TurboRFP.WPRE.RBG plasmid DNA.

CMV-driven TurboRFP expression. These AAV preparations are suitable purity for injection into animals.

Delivery

  • Volume100 µL
  • Titer≥ 1×10¹³ vg/mL
  • Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
  • StorageStore at -80℃. Thaw just before use and keep on ice.
  • ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

  • Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV1 cap gene
  • BufferPBS + 0.001% Pluronic F-68
  • SerotypeAAV1
  • PurificationIodixanol gradient ultracentrifugation
  • Reporter GeneTurboRFP

Biosafety

Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide

Resource Information

  • Terms and Licenses
    • Ancillary Agreement for Penn Vectors
    • Terms of Use for Viral Vectors
  • Industry Terms
    • Not Available to Industry

Viral Quality Control

Quality Control:
  • Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
  • Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Information for AAV5 (Catalog # 105548-AAV5)(Back to top)

Purpose

Ready-to-use AAV5 particles produced from pENN.AAV.CMVs.TurboRFP.WPRE.RBG (#105548). In addition to the viral particles, you will also receive purified pENN.AAV.CMVs.TurboRFP.WPRE.RBG plasmid DNA.

CMV-driven TurboRFP expression. These AAV preparations are suitable purity for injection into animals.

Delivery

  • Volume100 µL
  • Titer≥ 7×10¹² vg/mL
  • Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
  • StorageStore at -80℃. Thaw just before use and keep on ice.
  • ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

  • Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV5 cap gene
  • BufferPBS + 0.001% Pluronic F-68
  • SerotypeAAV5
  • PurificationIodixanol gradient ultracentrifugation
  • Reporter GeneTurboRFP

Biosafety

Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide

Resource Information

  • Terms and Licenses
    • Terms of Use for Viral Vectors
  • Industry Terms
    • Not Available to Industry

Viral Quality Control

Quality Control:
  • Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
  • Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Information for AAV8 (Catalog # 105548-AAV8)(Back to top)

Purpose

Ready-to-use AAV8 particles produced from pENN.AAV.CMVs.TurboRFP.WPRE.RBG (#105548). In addition to the viral particles, you will also receive purified pENN.AAV.CMVs.TurboRFP.WPRE.RBG plasmid DNA.

CMV-driven TurboRFP expression. These AAV preparations are suitable purity for injection into animals.

Delivery

  • Volume100 µL
  • Titer≥ 1×10¹³ vg/mL
  • Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
  • StorageStore at -80℃. Thaw just before use and keep on ice.
  • ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

  • Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV8 cap gene
  • BufferPBS + 0.001% Pluronic F-68
  • SerotypeAAV8
  • PurificationIodixanol gradient ultracentrifugation
  • Reporter GeneTurboRFP

Biosafety

Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide

Resource Information

  • Terms and Licenses
    • Ancillary Agreement for Penn Vectors
    • Terms of Use for Viral Vectors
  • Industry Terms
    • Not Available to Industry

Viral Quality Control

Quality Control:
  • Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
  • Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Addgene是一个非盈利性全球质粒共享信息库。它负责保存和提供质粒。 科学家发表文章如果涉及到质粒,会发一份到Addgene保存,其他科学家如果需要这个质粒,可以向Addgene索取。过去,中国科学家购买Addgene产品需要和Addgene美国总部联系,付款以及海关清关等都比较麻烦,为此,Addgene指定北京中原公司为其中国分销商,以便为中国科学家提供更好的服务。