联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | ||
|---|---|---|---|---|---|---|
| Plasmid | 141386 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75* | Add to Cart | |
| Lentiviral Prep | 141386-LV | Virus (1mL at titer ≥ 5x10⁶ TU/mL)and Plasmid.More Information | Add to Cart | |||
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Backbone
- Vector backbonepLVX-EF1alpha-IRES-Puro(Search Vector Database)
- Backbone manufacturerTakara Bio
- Backbone sizew/o insert(bp)8825
- Vector typeMammalian Expression, Lentiviral
- Selectable markersPuromycin
Growth in Bacteria
- Bacterial Resistance(s)Ampicillin
- Growth Temperature30°C
- Growth Strain(s)NEB Stable
- Copy numberUnknown
Gene/Insert
- Gene/Insert nameSARS-CoV-2-M
- SpeciesSARS-CoV-2
- Mutationhuman codon optimized
- Entrez GeneM(a.k.a. GU280_gp05)
- PromoterEF1alpha
- Tag/ Fusion Protein
- 2xStrep (C terminal on insert)
Cloning Information
- Cloning methodUnknown
Resource Information
- Supplemental Documents
- pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-Puro.gb
- Terms and Licenses
- UBMTA
- Industry Terms
- Industry MTA
Information for Lentiviral Prep (Catalog # 141386-LV)(Back to top)
Purpose
Ready-to-use Lentiviral Prep particles produced from pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-Puro (#141386). In addition to the viral particles, you will also receive purified pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-Puro plasmid DNA.
Lentiviral particles carrying SARS-CoV-2 membrane (M) protein. This virus is used to make stable cell lines expressing the SARS-CoV-2 membrane protein.Delivery
- Volume1mL
- Titer≥ 5x10⁶ TU/mL
- Pricing$100 USD for preparation of 1mL virus + $30 USD for plasmid.
- StorageStore at -80℃. Thaw just before use and keep on ice.
- ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.
Viral Production & Use
- Packaging PlasmidspsPAX2 (plasmid #12260)
- EnvelopepMD2.G (plasmid #12259)
- BufferOptiPro
- Selectable MarkerPuromycin
Biosafety
Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide
Resource Information
- Terms and Licenses
- Terms of Use for Viral Vectors
- Industry Terms
- Not Available to Industry
Viral Quality Control
Titering Method:
- ddPCR assay: 293T cells were transduced with serial dilutions of 141386-LV, harvested several days later, and genomic DNA was isolated. Copies of RRE were measured and normalized to RPP30.
Notes:
- PCR confirmation of insert: PCR was carried out on the viral preparation with primers targeting the pLenti backbone and the insert. The PCR product was visualized on an agarose gel for size confirmation.
Visit our viral production page for moreinformation.
