Purified egg avidin was conjugated to horse radish peroxidase (HRP) via reductive amination. Avidin has a strong affinity for biotin. Avidin HRP conjugates were found to be useful for the detection of materials with biotin residues.

Chicken egg

2 mg/mL in PBS and 50% glycerol

Binds to free biotin or biotinylated proteins.

ELISA, 1:30,000;western blot, 1:30,000;immunohistochemistry, 1:500

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