Protein A binds to the immunoglobulin G and M of many mammalian species. The product is protein A chemically linked to beaded agarose through a stable covalent linkage. The immobilized protein A on the beaded agarose could be utilized for the immunoprecipitation and purification of both polyclonal and monoclonal antibodies from mammalian species.

Formulation

10 mg;1 mL Agarose Beads suspended in 2 ml slurry in glycerol

10 mg protein A/mL matrix.The matrix linking chemistry is stable when eluted with weak acid (pH 2-3) and could be re-usable with no apparent loss of IgG binding capacity.

30-40 mg rabbit IgG/mL beads as tested using rabbit serum

IP, IAC

Protein A agarose consists of the native protein A immobilized on 4% cross linked agarose beads. It is made for the binding of immunoglobulins. Protein A is very heat and acid stable.

Product is stable for 30 days at ambient temperature For extended storage store product at 4 to–20ºC.

1. Trends Biochem. Sci. 1982.7: 74-76.doi:10.1016/0968-0004(82)90082-2

2. Rowan University School of Osteopathic Medicine. 2019. YfmK is a Novel Ne-lysine Acetyltransferase that Directly Acetylates the Histone-like Protein HBsu in Bacilius subtilis