Uracil DNA Glycosylase (UDG, UNG) is an engineered E. coli enzyme catalyzing the hydrolysis of the N-glycosidic bond between the uracil and sugar, leaving an apyrimidinic site in uracil-containing single or double-stranded DNA. For PCR diagnosis, UDG can be used for efficient removal of carryover contaminated uracil-containing DNA.

Application 

• Prevention of carryover contamination  
• Removing uracil-containing DNA
• Genotyping and Molecular Diagnosis

Enzyme activities: Hydrolyzes uracil-glycosidic bonds in single- and double-stranded DNA

Heat inactivation: +95°C for 5 minutes is sufficient for inactivation 

Storage buffer: 20mM Tris-HCl (pH8.0), 0.1mM EDTA-NaOH (pH 8.0), 50mM NaCl, 1mM DTT, 0.1ug/ul BSA, 50% Glycerol

Purity: ≥90% (SDS-PAGE)

DNase contamination test: Not detectable (Incubation with 10U enzyme and pUC19 plasmid at 37°C, 1hr) 

Activity test: Removal of uracil-containing PCR product with one unit of enzyme

Stability: 24 months at -20°C