HelixCript™ Easy cDNA Synthesis Kit is the mixed type of HelixCript™ 1st-Strand cDNA Synthesis Kit for easy preparation of RT reaction mixture. HelixCript™ Thermo Reverse Transcriptase and RNase Inhibitor are mixed as RT Enzyme Mix. 5X RT Reaction Mix contain the dNTP mix and DTT. This mixed formulation is designed to save time and reduce the error and contamination opportunites. HelixCript™ Easy cDNA Synthesis Kit provides the simple and fast preparation of RT reaction mixture for the efficient synthesis of first strand cDNA. 

 

Figure 1. RT-PCR using HelixCript™ 1st-Strand cDNA Synthesis Kit and random hexamer or oligo-dT as primers for reverse transcription. 1st-strand cDNA synthesis reactions were performed using the 300 ng total RNA isolated from human blood. 10% of each 1st-strand cDNA products were used in PCR reactions with primer sets corresponding to fragment of human dynein gene and 1.25 units HelixAmp™ Xtender DNA polymerase in 50 ul PCR reaction. 

Application 

• Generation of first-strand cDNA for subsequent PCR or Real-time PCR 
• cDNA library construction 

Contents 

• 50 pmole/㎕ oligo d(T20) primer
• 50 pmole/㎕ random hexamers 
• 5x RT Reaction Mix
• RT Enzyme Mix
• DEPC water 

Quality Control

HelixCript™ Easy cDNA Synthesis Kit was functionally tested for RT-PCR amplification on different sized products (0.5 ~ 5 kb) with 500 ng of total RNA.