HelixAmp^TM FastLAMP Kit is designed to provide a simple and faster solution for Loop-Mediated Isothermal Amplification (LAMP) of DNA targets. LAMP is an isothermal amplification technique that provides rapid detection of target nucleic acids. The 2x FastLAMP Buffer contains buffering reagents, dNTPs, Mg²⁺, salts, and proprietary enhancing agents. The FastLAMP Enzyme is a derivative of Bst DNA polymerase, large fragment.

HelixAmp^TM FastLAMP Kit allows the fast amplification of target within 30 minutes with appropriate LAMP primer set. 

Figure 1. Comparison of real-time fluorescence LAMP assays using HelixAmp™ FastLAMP kit and other competitors, derivatives of Bst DNA polymerase. The reaction mixtures contain a fluorescent intercalating dye for real-time monitoring of the DNA amplification and 1.0 fg of Lambda DNA (~20 copies) as template. HelixAmp™ FastLAMP k​it  shows the fastest amplification, and it reaches the plateau within 30 min reaction.  

Figure 2. Sensitivity comparison of HelixAmp™ FastLAMP Kit and a competitor (Bst 3.0 polymerase). LAMP reactions were performed with purified genomic DNAs from N. meningitidis serogroup W (genome size = about 2,270 kb) as a template, ranging from 0.4 to 4 x 10⁶ copies per reaction. After thirty minutes of reaction, the products were analyzed by an agarose gel electrophoresis. NTC, no template control.

Figure 3. Accuracy of the quantitative assay using HelixAmp™ FastLAMP kit. Serially diluted Lambda DNAs (2 x 10⁶ ~ 2 x 10¹ copies) were analyzed using the Lambda specific LAMP primer set and an intercalating fluorescent dye. The accuracy of quantification is demonstrated by R² value= 0.998.

Figure 4. LAMP reactions of positive samples (serially diluted lambda DNAs, 2 x 10⁶ ~ 2 x 10¹ copies) and no template control(NTC) using HelixAmp™ FastLAMP kit. The Tt (Threshold time) value of NTC reactions differs from that of positive reactions by about 20 minutes.

Application 

• Loop-Mediated Isothermal Amplification (LAMP)

Contents 

• FastLAMP Enzyme
• 2x FastLAMP Buffer
• D-solution