HelixCript™ Thermo Reverse Transcriptase, a Thermo stable and RNase H negative variant of M-MLV RTase, can synthesize cDNAs from purified RNA templates at a temperature range of 42℃ ~ 55℃ and especially shows the highest activity at 50℃. At the escalated temperature, the cDNA synthesis is enhanced partially due to the less internal structural formation of the template RNA and increased polymerization activity of RTase. The high processivity and productivity of HelixCript™ Thermo Reverse Transcriptase allows this enzyme to amplify the high yield of product and can synthesize cDNA of target gene up to 12 kb from RNA template. This enzyme is quite suitable to synthesize the first-strand cDNA for RT-PCR of target gene. The cDNA synthesis from total RNA or poly-(A)+ RNA is performed by random primer, oligo-d(T) primer, or gene-specific primer. 

Application

• Generation of first strand cDNA 
• RT- PCR, Real-time PCR 
• Primer extension analysis 
• RNA sequencing

Contents 

• HelixCript™ Thermo Reverse Transcriptase (200 units/㎕)
• 5X Reaction Buffer
• 0.1 M DTT
• 10 mM dNTP Mix (each 10 mM)

Quality Control

HelixCript™ Thermo Reverse Transcriptase is evaluated by activity of cDNA synthesis corresponding to transcript of human gene using total RNA isolated from human blood according to size up to 12 kb at 50℃.