- Overview
- Data/Specifications
- Literature/Support
- How To Use
- Related Products
Overview
Type III collagen is the second most abundant collagen in tissues and is found most commonly in tissues exhibiting elastic properties such as skin, lungs, intestinal walls and walls of blood vessels. It is a homotrimer comprised of three alpha-1 chains and resembles other fibrillar collagens in structure and function. It is synthesized as procollagen, similary to collagen I, but the N-terminal propeptide remains attached in the mature fibrillar type III form.
Mutations of type III collagen causes Ehlers-Danlos syndrome, EDS IV, which affect arteries, internal organs, joints and skin, and may cause sudden death when the large arteries rupture.
Symbols/Related Terms:
- COL3A1
- Collagen type III, alpha 1
- Collagen III, alpha-1 polypeptide
- collagen, type III, alpha 1 (Ehlers-Danlos syndrome type IV, autosomal dominant)
- EDS4A
Data/Specifications
Description: Purified Bovine Collagen Type III (Atelocollagen) from bovine placenta
Form: Lyophilized, salt free, 10 mg/ vial
Sterility: Passed sterility test for bacteria and fungi
Source: Bovine placenta
Purity: Bovine Collagen Type III (Atelocollagen) > 90% / Bovine Collagen Type I (Atelocollagen) < 10%
Purification: Partial pepsin digestion in acidic conditions and differential salt precipitation.
Reconstitution: Dissolve in 10-50 mM acetic acid. Suspension should be shaken or stirred for several hours or overnight between 2-8°C. Material is dissolved when it appears homogeneous, with no schlieren pattern (light refraction due to differing protein concentrations in the suspension). Recommended concentration 1-5 mg/ml.
Storage: Bovine Collagen Type III (Atelocollagen) dissolved in acetic acid is stable at 4°C for 1 month. Lyophilized collagen long term storage (2 years) at -20°C or lower.
Application: Coating material for cell culture studies or 3D gel formation.
Literature/Support
Bovine Collagen Type III (Atelocollagen) Insert (PDF)
Note: inserts are for review only. Please refer to the insert shipped with your product to ensure the most up-to-date revision is being used.
How To Use
Dissolve in 10-50 mM acetic acid. Suspension should be shaken or stirred for several hours or overnight between 2-8°C. Material is dissolved when it appears homogeneous, with no schlieren pattern (light refraction due to differing protein concentrations in the suspension). Recommended concentration 1-5 mg/ml.
