- Overview
- Data/Specifications
- Literature/Support
- How It Works
- Related Products
Overview
An enkephalin is a pentapeptide involved in regulating nociception in the body. The enkephlins are termed endogenous ligands, or specifically endorphins, as they are internally derived and bind to the body"s opiod receptors. They function as neurotransmitters or neuromodulators and inhibit neurotransmitters in the pathway for pain perception. There are two forms of enkephalin, one containing leucine, and the other containing methionine. These two neuropeptides can depress neurons throughout the central nervous system. Although it is not known exactly how these neuropeptides function, the enkephalins are natural pain killers and may be involved, wth other neuropeptides, in the development of psychopathologic behavior in some cases.
Data/Specifications
Species: human, mouse, rat
Sample Type:serum, plasma, urine, cell culture supernate, tissue homogenates, CSF
Sample Preparation:plasma extraction
Sample Size:50 uL
Standard Curve Range: 0.01 - 100 ng/mL
Sensitivity: 0.5 ng/mL
Assay Length: 4 hrs
Cross-reactivity
Leu-Enkephalin: 100 %
MET-Enkephalin: 0 %
MET-Enkephalin-Arg-Gly-Leu: 0 %
Leu-Enkephalin-Arg: 0 %
Dynorphin A: 30 %
beta-Endorphin (Human): 0 %
Literature/Support
Product Insert:
Leu-Enkephalin ELISA Insert (PDF)
Articles/Troublshooting:
ELISA Troubleshooting Guide
ELISA Data Reduction Guide
How It Works
The immunoplate in this kit is pre-coated with secondary antibody and the nonspecific binding sites are blocked. The secondary antibody can bind to the Fc fragment of the primary antibody (peptide antibody) whose Fab fragment will be competitively bound by both biotinylated peptide and peptide standard or tar- geted peptide in sample. The biotinylated peptide is able to interact with streptavidin-horseradish peroxidase (SA-HRP) which catalyzes the substrate solution composed of 3,3’,5,5’-tetramethylbenzidine (TMB) and hydrogen peroxide to produce a blue colored solution. The enzyme-substrate reaction is stopped by hydrogen chloride (HCl) and the solution turns to yellow. The intensity of the yellow is directly proportional to the amount of biotinylated peptide-SAHRP complex but inversely proportional to the amount of the peptide in standard solutions or samples. This is due to the competitive binding of the biotinylated peptide and the peptide in standard solutions or samples to the peptide antibody (primary antibody). A standard curve of a peptide with known concentration can be established accordingly. The peptide with unknown concentration in samples can be determined by extrapolation to this standard curve.
