ProductDescription
YO-01027(Dibenzazepine,DBZ)isadipeptidicg-secretaseinhibitorwithIC50of2.6and2.9nMforAPPLandNotch,respectively.YO-01027targetsthePresenilinfragment.IncreasingconcentrationsofYO-01027admiNISTeredtoAPPl-orNotch-expressingcellsleadstotheprogressiveaccumulationofAPPLC-terminalfragmentsandadecreaseinNICDproductioninadose-dependentmanner.[1]
Inaninvitromodelofhumancornealandconjunctivalepithelialcelldifferentation,YO-01027impairedMUC16biosynthesisinaconcentration-dependentmanner.[2]
Inoncologymodels,YO-01027preferentiallyinhibitedNotchandsignificantlydecreasedMCF7tumorsandincreasedlatencycomparedwithcontrolmice.[3]
Technicalinformation:
| ChemicalFormula: | C26H23F2N3O3 | |
| CAS#: | 209984-56-5 | |
| MolecularWeight: | 463.48 | |
| Purity: | >98% | |
| Appearance: | White | |
| ChemicalName: | 7-(S)-[N"(3,5-difluorophenylacetyl)-L-alaninyl]amino-5-methyl-5,7-dihydro-6H-dibenz[b,d]azepin-6-one | |
| Solubility: | Upto100mMinDMSO | |
| Synonyms: | YO-01027,YO01027,Dibenzazepine,DeshydroxyLY411575 |
ShippingCondition:Theproductisshippedinaglassvialatambienttemperature.
Storagecondition:Forlongershelflife,storesolidpowderat4oCdesiccated,orstoreDMSOsolutionat-20oC.
Reference:
| 1. | Grothetal.,PharmacologicalAnalysisofDrosophilamelanogasterg-SecretasewithRespecttoDifferentialProteolysisofNotchandAPP.Mol.Pharmacol.2010,77(4),567-574.PubmedID:20064975 |
| 2. | Xiongetal.,NotchSignalingModulatesMUC16BiosynthesisinanInVitroModelofHumanCornealandConjunctivalEpithelialCellDifferentiation.Invest.Ophthalmol.Vis.Sci.2011,52(8),5641-5646.PubmedID:21508102 |
| 3. | Harrisonetal.,RegulationofBreastCancerStemCellActivitybySignalingthroughtheNotch4Receptor.CancerRes.,2010,70,709-718.PubmedID:20068161 |
Cellagen的FlexiWash FW400自动膜/凝胶洗涤站设计用于自动Western blot膜洗涤和孵育过程。将蛋白质转移到膜上后，将膜加载到FlexiWash上以进行自动封闭，孵育和洗涤循环。用户只需要准备所需的试剂，启动印迹程序，然后让FW400进行其余操作即可。凭借独特的液体处理设计，FW400同时支持四个清洗/孵育过程，每个过程均可独立编程，并针对特定应用实现全自动。FW400也可用于蛋白质或DNA凝胶染色和脱色，或涉及重复液体处理和孵育周期的任何其他应用。
