ProductDescription
Bortezomibisafirst-in-classdipeptideboronicacid-based,water-solubleproteasomeinhibitor.Asasingleagent,bortezomibwasfoundtohaveconsistentantitumoractivityinbothchemosensitiveandchemoresistantmultiplemyelomacellsatanIC50of10-20ng/mL.[1]BortezomibovercomestheresistancetoapoptosisinmultiplemyelomacellsthatisinducedbyIL-6.[2]Additionally,bortezomibpreventsTNF-a-induced,NF-kB-dependentupregulationofIL-6andreducescelladhesion;proliferationofremainingadherentmultiplemyelomacellswasalsoinhibitedbybortezomib.[1]
InMMcelllinesU266,IM-9,andHsSultan,bortezomibinhibitedatIC50concentrationsof3,6,and20nM,respectively.[2]CellgrowthofDox40,MR20,andLR5MMcellswascompletelyinhibitedbybortezomibat100nMIC50.
BortezomibsuppressesgrowthandinducesapoptosisinBcr/Abl-positivecellssensitiveandresistanttoIM.Interestingly,sequentialcombinationofbortezomibfollowedbyimatinibresultedinasynergisticpro-apoptoticeffectinimatinib-resistantcells;simultaneousexposureofbortezomibandimatinibwasantagoNISTic.[3]
Technicalinformation:
| ChemicalFormula: | C19H25BN4O4 | |
| CAS#: | 179324-69-7 | |
| MolecularWeight: | 384.24 | |
| Purity: | >98% | |
| Appearance: | LightYellowCrystalline | |
| ChemicalName: | [(1R)-3-methyl-1-({(2S)-3-phenyl-2-[(pyrazin-2-ylcarbonyl)amino]propanoyl}amino)butyl]boronicacid | |
| Solubility: | Upto100mMinDMSO | |
| Synonyms: | PS-341,PS341,Bortezomib,Velcade,LDP-341 |
ShippingCondition:Theproductisshippedinaglassvialatambienttemperature.
Storagecondition:Forlongershelflife,storesolidpowderat4oCdesiccated,orstoreDMSOsolutionat-20oC.
Reference:
| 1. | Richardsonetal.,CancerControl,2003,10(6),361-369. |
| 2. | Hideshimaetal.,TheproteasomeinhibitorPS-341inhibitsgrowth,inducesapoptosis,andovercomesdrugresistanceinhumanmultiplemyelomacells.CancerRes.2001,61,3071-3076.PubmedID:1306489 |
| 3. | Gattoetal.,TheproteasomeinhibitorPS-341inhibitsgrowthandinducesapoptosisinBcr/Abl-positivecelllinessensitiveandresistanttoimatinibmesylate.Haematologica,2003,88(8),853-863.PubmedID:12935973 |
Cellagen的FlexiWash FW400自动膜/凝胶洗涤站设计用于自动Western blot膜洗涤和孵育过程。将蛋白质转移到膜上后，将膜加载到FlexiWash上以进行自动封闭，孵育和洗涤循环。用户只需要准备所需的试剂，启动印迹程序，然后让FW400进行其余操作即可。凭借独特的液体处理设计，FW400同时支持四个清洗/孵育过程，每个过程均可独立编程，并针对特定应用实现全自动。FW400也可用于蛋白质或DNA凝胶染色和脱色，或涉及重复液体处理和孵育周期的任何其他应用。
