SB-431542 is a selective and potent inhibitor of activin receptor-like kinase (ALK, the TGF-β type I receptor), specifically ALK4, ALK5 (IC50 = 94 nM) and ALK7. It has no effect on the other, more divergent ALK family members that recognize bone morphogenetic proteins (BMPs) such as ALK2, ALK3 or ALK6. It has also no effect on components of the ERK, JNK, or p38 MAP kinase pathways or on components of the signaling. SB431542 specifically blocks Smad signaling and suppresses renewal in embryonic and induced pluripotent stem (iPS) cells and promotes differentiation. SB431542 could enhance reprogramming efficiency when using it with MEK inhibitor PD0325901.
Technical information:
| Chemical Formula: | C22H16N4O3 | |
| CAS #: | 301836-41-9 | |
| Molecular Weight: | 384.39 | |
| Purity: | >98% | |
| Appearance: | Off white solid | |
| Chemical Name: | 4-(4-(benzo[d][1,3]dioxol-5-yl)-5-(pyridin-2-yl)-1H-imidazol-2-yl)benzamide | |
| Solubility: | Up to 100 mM in DMSO |
Shipping Condition: The product is shipped in a glass vial at ambient temperature.Storage: For longer shelf life, store solid powder at 4oC desiccated, or store DMSO solution at -20oC.
Reference:
| 1. | Tongxiang Lin, et al. A chemical platform for improved induction of human iPSCs. Nature Methods 6, 805 - 808 (2009). |
| 2. | Laping NJ, et al. Inhibition of transforming growth factor (TGF)-beta1-induced extracellular matrix with a novel inhibitor of the TGF-beta type I receptor kinase activity: SB-431542. Mol Pharmacol. 2002; 62(1):58-64. |
| 3. | Inman GJ, et al. SB-431542 is a potent and specific inhibitor of transforming growth factor-beta superfamily type I |
Other Information:
Product Specification (pdf)MSDS (pdf)
Cellagen的FlexiWash FW400自动膜/凝胶洗涤站设计用于自动Western blot膜洗涤和孵育过程。将蛋白质转移到膜上后，将膜加载到FlexiWash上以进行自动封闭，孵育和洗涤循环。用户只需要准备所需的试剂，启动印迹程序，然后让FW400进行其余操作即可。凭借独特的液体处理设计，FW400同时支持四个清洗/孵育过程，每个过程均可独立编程，并针对特定应用实现全自动。FW400也可用于蛋白质或DNA凝胶染色和脱色，或涉及重复液体处理和孵育周期的任何其他应用。
