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蚂蚁淘在线 / 品牌中心 / Cellagen / Cellagentech/Repro-ThiPSC | Enhance reprogramming efficiency cocktail/K8447-500/0.5mL of 1000x
商品描述
ProductDescription
Repro-ThiPSC(i.e.SMC4inreference1)isacocktailoffoursmallmoleculesofspecificsignalingpathwayinhibitors.ItcanbeaddeddirectlyinmediatoincreasereprogrammingefficiencyofsomaticcellstoiPSCs,andtoimproveexpansionandmaintenanceofhiPSCsunderbothwithfeedercellsandfeeder-freeconditions.Itcanbeusedincombinationwithanyofthewidelyusedreprogramminggeneexpressionsystems(e.g.retro/lenti-viral,episomal,mRNA,orproteins)toenhancethereprogrammingsomaticcellstoiPScells.Thisprocessisrobust,efficientandcanbeeasilyscaled-up1-3.
Technicalinformation:
| CocktailFormulation: 1000xDMSOstocksolution | 5mMofThiazovivin(ROCKinhibitor), 0.4mMofPD325901(MEKinhibitor), 2mMofSB431542(TGF-ß1inhibitor),and 1mMofCHIR99021(GSK3ßinhibitor) | |
| Purity: | >98%(foreachcompound) |
ShippingCondition:Theproductisshippedinaglassvialatambienttemperature.
Storage:StoreDMSOsolutionat-20oC.
Usage:DiluteRepro-ThiPSCstocksolution(1000x)at1:1000intobasiciPSCinductionmediatomake1xRepro-ThiPSCmedia.
Reference:
| 1. | ValamehrB,etal.Anovelplatformtoenablethehigh-throughputderivationandcharacterizationoffeeder-freehumaniPSCs.ScientificReports.2012.2:213. |
| 2. | LiW,etal.Generationofhuman-inducedpluripotentstemcellsintheabsenceofexogenousSox2.StemCells.2009.27(12):2992-3000. |
| 3. | KawamataM,etal.Generationofgeneticallymodifiedratsfromembryonicstemcells.PNAS.2010.107,14223-14228. |
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