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商品描述
| Product information | Key steps | References | Technical support | Reviews |
Product overview
| Product name | L-Kynurenine ELISA kit |
| Description | Enzyme immunoassay (ELISA) for the quantitative determination of L-Kynurenine in biological fluids and cell supernatant. |
| Format | 96-well plate |
| Samples | Plasma, serum, cell culture supernatant |
| Minimal sample volume | 20 µL |
| Cross specie-reactivity | React with all species |
| Standard range | 0/100 - 10000 ng/ml |
| Sensitivity | < 47.5 ng/ml |
| Specificity | No significant cross-reactivity was observed with L-Kynurenine analogs such as L-Tryptophan, 3-Hydroxy-DL-Kynurenine, 5-Hydroxy-DL-Tryptophan, Tyrosine, Serotonin, Phenylalanin, L-Asparagine and Kynurenic acid. |
| Assay time | Sample acylation 90 min and ELISA overnight |
| Storage | Store at 2-8°C for to 6 months. |
| Datasheets | Instructions for use, Material Safety Datasheet |
Key steps
| Sample collection & storage | Plasma/Serum: Do not use lipemic, haemolytic samples, as well as samples containing precipitates or fibrin strands. Store smaples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months). |
| Sample preparation | Sample acylation (90 min) |
| ELISA | L-Kynurenine antiserum overnight incubation, revelation and read steps (1h). |
| Detailed protocol | Download instructions for use |
References
Product citations :
- Klar et al. A highly efficient modality to block the degradation of tryptophan for cancer immunotherapy: locked nucleic acid-modified antisense oligonucleotides to inhibit human indoleamine 2,3-dioxygenase 1/tryptophan 2,3-dioxygenase expression. Cancer Immunol Immunother. 2020 Jan
- Watanabe et al. Adding indoximod to hypofractionated radiotherapy with anti-PD-1 checkpoint blockade enhances early NK and CD8+ T cell-dependent tumor activity. Clin Cancer Res. 2019 Nov 6
- Hong et al. Physical exercise prevents mice from L-Kynurenine-induced depression-like behavior. Asian J Psychiatr. 2019 Dec 19
- Allison et al. Exercise training impacts skeletal muscle gene expression related to the kynurenine pathway. Am J Physiol Cell Physiol. 2019 Mar 1
- Qi et al. Lactobacillus maintains healthy gut mucosa by producing L-Ornithine Commun Biol. 2019 May 8
- Walker et al. Leucine competes with kynurenine for blood-to-brain transport and prevents lipopolysaccharide-induced depression-like behavior in mice. Mol Psychiatry. 2018 Jul 9.
- Ziklo et al. Dysbiosis of the Vaginal Microbiota and Higher Vaginal Kynurenine/Tryptophan Ratio Reveals an Association with Chlamydia trachomatis Genital Infections. Front Cell Infect Microbiol. 2018 Jan 18.
- Gautam et al. In vivo inhibition of tryptophan catabolism reorganizes the tuberculoma and augments immune-mediated control of Mycobacterium tuberculosis. Proc Natl Acad Sci U S A. 2018 Jan 2
- Martiin-Hernandez et al. Chronic Mild Stress Alters Kynurenine Pathways Changing the Glutamate Neurotransmission in Frontal Cortex of Rats. Mol Neurobiol. 2018 May 3
- Toulmonde et al. Use of PD-1 Targeting, Macrophage Infiltration, and IDO Pathway Activation in Sarcomas. JAMA Oncol. 2018 Jan 1;4
- Lee et al. Inhibition of acute lethal pulmonary inflammation by the IDO–AhR pathway. Proc Natl Acad Sci U S A. 2017 Jul 18
- Maliniemi et al. Biological and clinical significance of tryptophan-catabolizing enzymes in cutaneous T-cell lymphomas. Oncoimmunology. 2017; 6
- Escobar-Alvarez et al. The peroxyl radical-induced oxidation of Escherichia coli FtsZ and its single tryptophan mutant (Y222W) modifies specific side-chains, generates protein cross-links and affects biological function. Free Radic Biol Med. 2017 Nov
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