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商品描述
| Product information | Key steps | References | Technical support | Reviews |
Product overview
| Product name | Norepinephrine-Dopamine ELISA kit - Research |
| Description | Enzyme immunoassay (ELISA) for the ultra-sensitive determination of dopamine and noradrenaline in biological samples. Research use only. |
| Format | 2 x 96-well plate |
| Samples | Any sample |
| Sample volume | 1 - 750 µl |
| Cross specie-reactivity | React with all species |
| Standard range | Norepinephrine: 0 / 0.2 - 32 ng/mlDopamine: 0 / 0.5 - 80 ng/ml |
| Sensitivity | Norepinephrine: 2.6 pg/mlDopamine: 6.6 pg/ml; |
| Specificity | No significant cross-reactivity was observed with Dopamine and Norepinephrine analogs such as Epinephrine, Metanephrine, Normetanephrine, 3-Methoxytyramine, 3-Methoxy-4-hydroxyphenylglycol, Tyramine, Phenylalanine, Caffeinic acid, L-Dopa, Homovanillic acid, Tyrosine and 3-Methoxy-4-hydroxymandelic acid. |
| Assay time | Sample preparation 4 hours and ELISA overnight |
| Storage | Store at 2-8°C for to 6 months. |
| Datasheets | Instructions for use , Material safety datasheet |
Key steps
| Sample preparation | Sample extraction and acylation (1h45), enzymatic conversion (2h) |
| ELISA | Dopamine/Norepinephrine antiserum overnight incubation, revelation and read steps (1h). |
| Detailed protocol | Download instructions for use |
References
- Sontag W & Kalka D. No effect of pulsed electromagnetic fields on PC12 and HL-60 cells. Radiat Environ Biophys. 2006 May;45(1):63-71.
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