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商品描述
| Product information | Key steps | References | Technical support | Reviews |
Product overview
| Product name | Epinephrine-Norepinephrine ELISA kit - Research |
| Description | Enzyme immunoassay (ELISA) for the ultra-sensitive determination of epinephrine and norepinephrine in biological samples. Research use only. |
| Format | 2 x 96-well plate |
| Samples | Any sample |
| Sample volume | 1 - 750 µl |
| Cross specie-reactivity | React with all species |
| Standard range | Epinephrine: 0 / 0.5 - 80 ng/ml;Norepinephrine: 0 / 0.2 - 32 ng/ml |
| Sensitivity | Epinephrine: 6.6 pg/ml; Norepinephrine: 2.6 pg/ml |
| Specificity | No significant cross-reactivity was observed with Epinephrine and Norepinephrine analogs such as Dopamine, Metanephrine, Normetanephrine, 3-Methoxytyramine, 3-Methoxy-4-hydroxyphenylglycol, Tyramine, Phenylalanine, Caffeinic acid, L-Dopa, Homovanillic acid, Tyrosine and 3-Methoxy-4-hydroxymandelic acid. |
| Assay time | Sample preparation 4 hours and ELISA overnight |
| Storage | Store at 2-8°C for to 6 months. |
| Datasheets | Instructions for use , Material safety datasheet |
Key steps
| Sample preparation | Sample extraction and acylation (1h45), enzymatic conversion (2h) |
| ELISA | Epinephrine/Norepinephrine antiserum overnight incubation, revelation and read steps (1h). |
| Detailed protocol | Download instructions for use |
References
- El Khoury N et al. Upregulation of the hyperpolarization-activated current increases pacemaker activity of the sinoatrial node and heart rate during pregnancy in mice. Circulation. 2013 May 21;127(20):2009-20.
- Jun JC et al. Acute hypoxia induces hypertriglyceridemia by decreasing plasma triglyceride clearance in mice. Am J Physiol Endocrinol Metab. 2012 Aug 1;303(3):E377-88.
- Al-Wadei M. and Schuller HM. Pancreatic cancer cells and normal pancreatic duct epithelial cells express an autocrine catecholamine loop that is activated by nicotinic acetylcholine receptors α3, α5 and α7. Mol Cancer Res. Feb 2012; 10(2): 239–249.
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