Product Specifications:

Item# 20050: Recombinant Malaria  pf HRP II Antigen (E.coli)                    

Concentration:See vial                                                         

Mass/vial:  See vial                                                                                        

Diluent: 50mM Tris, pH8, 2mM DTT  0.02%SDS                                                                         

Purity: >95%                                                                       

Stabilizer: None                                                                        

Preservative: None

Storage: -75°C

Physical State: Frozen Liquid

Stability: At least 36 months. 

Application: ELISA, Western ELISA, Diagnostics.

Description: Synthetic malaria parasite pf HRP II DNA sequence, expressed as C-terminus 4 his protein in the E.coli Expression System

Purification:This protein was purified by aqueous solvent extraction Ni-affinity and preparative electrophoresis to  >98% purity as determined by SDS-PAGE, reduced.

Molecular Weight: approx. 40kD.

Specificity: This protein binds to Anti HRP II  murine monoclonal antibodies IgG and IgM and to malaria parasite pf HRP II converted human sera as determined by Elisa and  Western Blots assays. The antigen is suitable for one-step lateral flow diagnostic testing.

Application and Instructions for use

Recommended concentrations for use are approximate values.  Elisa is performed in 1-2ug/ml protein range for titering Dengue virus converted human polyclonal antibodies. Concentarions if 1-2mg/ml (1-2ul/cm) are recommended for Lateral Flow Diagnostics.