PRODUCT SPECIFICATION

Product# 1121:  Murine anti-gp120 HIV-1  Monoclonal Antibody

                                            (Cross Reactive)

SPECIFICATIONS                                            APPLICATIONS

  Antibody Class: IgG2b                                    ELISA, Western ELISA

  Concentration: See vial                                   Immunoprecipitation

  Mass/vial: 100µg                                             Immunocytochemistry

  Purity: >95%                                                   Immunohistochemistry

  Stabilizer: None                                               In Vitro Virus Neutralization

  Preservative: None  

  Physical State: Frozen Liquid

  Storage: -75oC

  Stability: At least 12 months at -75oC

DESCRIPTION: High affinity murine anti-gp120 HIV-1 mAb. Epitope: 315 to 330 amino acids of HIV-1 gp120.

SPECIFICITY: Binds to V3-loop of HIV-1 gp120/gp160.  Cross reactive with MN, IIIB, RF, SF2 and other primary HIV-1 isolates.

BIOLOGICAL ACTIVITY: Neutralizes different strains of HIV-1 at 1-10µg/ml as determined by syncycium assays with CD4-bearing HIV-1 infected cells and co-cultures of HIV-1 infected human PBLs.

APPLICATIONS AND INSTRUCTIONS FOR USE

Recommended dilutions for use are approximate values.  A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.

ELISA may be performed at 10-100ng/ml of mAb depending on the amount of immobilized antigen in the assays.  Western ELISA require at 1-5µg/ml mAb..