ProductName:Goat Anti-Rabbit IgG Antibody (H&L) HRP

Target Species:Rabbit IgG

Tested applications:IHC-P, WB, ELISA

Immunogen:Rabbit IgG, whole moleculeConjugation:HRP

Applications

WB:1/3000-1/20000

IHC:1/100-1/2000

ELISA: 1/5000-1/10000

Working dilutions are highlighted in the table below,use at an assay dependent dilution.

Chemiluminescence systems have emerged as the best all-around method for western blot detection.They

eliminate thehazards associated with radioactive materials and toxic chromogenic substrates. The speed and

sensitivity of these methodsare unequalled by traditional alternatives, and because results are generated on

film, it is possible to record and store datapermanently. Blots detected with chemiluminescentmethods are

easily stripped for subsequent reprobing with additional antibodies.HRP-conjugated secondary antibodies are

utilized in conjunction with specific chemiluminescent substrates to generate the light signal.HRPconjugates

have a very high turnover rate, yielding good sensitivity with short reaction times.