Description:Mouse monoclonal antibody against N-terminalsequence of human amyloid beta peptides

Purification:Protein G affinity purified

Product Type: Primary antibody,

Target Protein:Human beta amyloid peptide 42

Immunogen:Full length human beta amyloid peptide 42

Fusion Myeloma:Sp2/0-Ag14

Specificity:This antibody recognizes the N-terminal peptide (DAEFRHDS) of human beta amyloid peptides and also reacts with full length Aβ40, Aβ42 and Aβ43.

Species Reacitvity:Human

Host / Isotype: Mouse, IgG1 Kappa

Formulation:Lyophilized from a solution in 0.01M PBS pH7.2

Reconstitution:Double distilled water is recommended to adjust the final concentration to 1.00mg/mL.

Storage: Store at -20^oC

Research Area:Aging and neurodegenerative diseases

Background:

Amyloid beta peptides Aβ42 and Aβ40 have been investigated extensively for predicating Alzheimer’s disease. A recent study on amyloid beta peptide Aβ43 in brain showed that Aβ43 is more fibrillogenic than other amyloid beta peptides and could be more useful as a biomarker or therapeutic target for Alzheimer’s disease.

Since Aβ40, Aβ42 and Aβ43 are different only at the few C-end amino acids, antibody to N-terminal sequence can bind with all three amyloid beta peptides.

Application:

1. ELISA:

The antibody was used as capture antibody in sandwich ELISA for Aβ42 detection in combination with detection antibody CA9 10C11 (Cat. No.: MO-M40093B).

The antibody was used as capture antibody in sandwich ELISA for Aβ40 detection in combination with detection antibody CV9 7B10 (Cat. No.: MO-M40095A).

2. IHC: The picture below shows the 40x amplified brain tissue section from transgenic Alzheimer’s disease mouse model after IHC staining with clone NT 4A2.

(Procedure: After heat induced epitope retrieval, formalin-fixed paraffin-embedded brain tissue section from 12 month old APPswe/PSEN1dE9 mouse was incubated at 4^oC with 0.01mg/ml NT 4A2 overnight. Bound antibody was detected using a HRP-labeled goat anti-mouse antibody).

References:

If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude.