Instant ELISA Kit for Interferon Alpha (IFNa)
IFNA1; IFL; LeIF D; IFN; IFN-Alpha; IFNA13; IFN-A; IFNAP22; IFN-Alpha 1b; Interferon Alpha 1b; Interferon, Leukocytic; IFN, Leukocyte; Interferon alpha-D
- Product No.IEA033Ga
- Organism SpeciesChicken (Gallus) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Caprine
- Ovine
- Equine
- Bovine
- Porcine
- Gallus
- Canine
- Others
- Multi-species
- Pan-species
- Test MethodDouble-antibody Sandwich
- Assay Length1h, 10min
- Detection Range15.6-1,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 5.4pg/mL.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- DownloadInstruction Manual
- UOM48T96T96T*596T*1096T*100
- FOBUS$ 718 For more details, please contact local distributors!US$ 1026 For more details, please contact local distributors!US$ 4617 For more details, please contact local distributors!US$ 8721 For more details, please contact local distributors!US$ 71820 For more details, please contact local distributors!
Packages (Simulation)- Packages (Simulation)
- Results demonstration
- Typical Standard Curve
- ISO9001: 2008, ISO13485: 2003 Registered
Specificity of the Instant ELISA Kit for Interferon Alpha (IFNa)
This assay has high sensitivity and excellent specificity for detection of Instant Interferon Alpha (IFNa).No significant cross-reactivity or interference between Instant Interferon Alpha (IFNa) and analogues was observed.
Recovery of the Instant ELISA Kit for Interferon Alpha (IFNa)
Matrices listed below were spiked with certain level of recombinant Instant Interferon Alpha (IFNa) and the recovery rates were calculated by comparing the measured value to the expected amount of Instant Interferon Alpha (IFNa) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 83-97 | 93 |
| EDTA plasma(n=5) | 80-92 | 85 |
| heparin plasma(n=5) | 79-97 | 90 |
Precision of the Instant ELISA Kit for Interferon Alpha (IFNa)
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Instant Interferon Alpha (IFNa) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Instant Interferon Alpha (IFNa) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10%>Inter-Assay: CV<12%>
Linearity of the Instant ELISA Kit for Interferon Alpha (IFNa)
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Instant Interferon Alpha (IFNa) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 88-102% | 97-105% | 89-96% | 98-105% |
| EDTA plasma(n=5) | 82-95% | 78-101% | 95-105% | 87-99% |
| heparin plasma(n=5) | 86-103% | 89-97% | 80-94% | 79-90% |
Stability of the Instant ELISA Kit for Interferon Alpha (IFNa)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary of the Instant ELISA Kit for Interferon Alpha (IFNa)
1. Prepare all reagents, samples and standards;2. Add 100µL standard or sample to each well. Incubate 30 minutes at 37°C;3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 30 minutes at 37°C;4. Aspirate and wash 3 times;5. Add 100µL prepared Detection Reagent B. Incubate 10 minutes at 37°C;6. Aspirate and wash 5 times;7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;8. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle of the Instant ELISA Kit for Interferon Alpha (IFNa)
The test principle applied in this kit is enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Instant Interferon Alpha (IFNa). Standards or samples and HRP-labeled detection antibody specific to Instant Interferon Alpha (IFNa) (Detection Reagent A) are then added to the appropriate microtiter plate wells. Next, TMB substrate solution is added, only those wells that contain Instant Interferon Alpha (IFNa), and HRP-labeled detection antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Instant Interferon Alpha (IFNa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
GIVEAWAYS
ELISA / CLIA Experiment Service
INCREMENT SERVICES
Single-component Reagents of Assay KitLysis Buffer Specific for ELISA / CLIAQuality Control of ELISA / CLIADisease Model Customized ServiceTGFB1 Activation ReagentReal Time PCR Experimental Service
Related products
| Catalog No. | Organism species: Chicken (Gallus) | Applications (RESEARCH USE ONLY!) |
| RPA033Ga01 | Recombinant Interferon Alpha (IFNa) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA033Ga01 | Polyclonal Antibody to Interferon Alpha (IFNa) | WB; IHC; ICC; IP. |
| MAA033Ga21 | Monoclonal Antibody to Interferon Alpha (IFNa) | WB; IHC; ICC; IP. |
| SEA033Ga | ELISA Kit for Interferon Alpha (IFNa) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| IEA033Ga | Instant ELISA Kit for Interferon Alpha (IFNa) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA033Ga | CLIA Kit for Interferon Alpha (IFNa) | Chemiluminescent immunoassay for Antigen Detection. |
| KSA033Ga01 | ELISA Kit DIY Materials for Interferon Alpha (IFNa) | Main materials for "Do It (ELISA Kit) Yourself". |
