Description

The ExcelTaq™ 5X PCR Master Dye Mix is a ready-to-use mixture for amplifying targeted DNA fragments. It is designed to serve as ready-to-use master mix for virtually all PCR applications. The mixture contains all components for PCR with the exception of template and primers. This not only saves valuable time in the laboratory, but also reduces the number of pipetting and reagent handling errors. The PCR Master Dye Mix is supplied as a 5X concentrated ready-to-use mix, that is a mixture of recombinant Taq DNA polymerase, reaction buffer, MgCl2, dNTP, enzyme stabilizer and PCR-friendly loading dye solution containing tracking dye (Bromophenol blue) enabling efficient amplification of template in PCR and allows the user to prepare a PCR reagent – loading dye master mix conveniently.

Features

• 5’→3’ DNA polymerase activity

• No detectable 3"→5" exonuclease (proofreading) activity

• Generates PCR products with 3"-dA overhangs

• High yield PCR 

• High reproducibility

• Reduced  pipetting errors

• Includes tracking dye for direct loading after PCR

Applications 

• Routine PCR

• Colony PCR

• High throughput PCR

• Amplification of DNA fragments up to 8 kb

• Generation of PCR products for TA cloning

• DNA labeling