Description
The ExcelRT™ One-Step RT-PCR Kit is designed for the reverse transcription and PCR amplification of a specific target RNA from either total RNA or mRNA. The ExcelRT™ One-Step RT-PCR Kit provides the user an alternative to the lengthy two step processes (first strand generation and amplification) by using a single mixture, single tube, one step reaction. The ExcelRT™ One-Step RT-PCR Kit contains a 2X reaction premix consisting of an optimized buffer, dNTPs, Mg2+ and enzyme stabilizer, and a blend of recombinant reverse transcriptase and Taq DNA polymerase. The ExcelRT™ One-Step RT-PCR Kit allows the user to complete the RT-PCR process using a thermocycler in a single reaction setting. The ExcelRT™ One-Step RT-PCR Kit is capable of detecting even trace amounts of target RNA and ideal for target RNA amplification and analysis
Features
Generates dsDNA directly from RNA samples
High sensitivity and yields
High reproducibility, reduced pipetting errors
High throughput
Reduced RNase H ribonuclease activity
Storage
-20°C for 24 months
Contents
Storage -20°C for 24 months |
High sensitivity and yields
Manual
Manual_RP1100_ExcelRT™ 2X One-Step RT-PCR Kit
SDS
SDS_RP1100
[RP1000] ExcelRT™ Reverse Transcriptase
High yield
Thermostable, up to 50°C, during first strand synthesis
High processivity, generating cDNA up to 8 kb
Reduced RNase H ribonuclease activity
[RI1000] RNAok™ RNase Inhibitor
Application
RT-PCR
cDNA Synthesis
in vitro transcription
[NS1000] FluoroVue™ Nucleic Acid Gel Stain
Excellent for in-gel staining
Sensitivity up to 0.14 ng DNA or 1 ng total RNA
A safer alternative to EtBr
Suitable to blue or UV light
Excellent for in-gel staining
Sensitivity up to 0.14 ng DNA or 1 ng total RNA
A safer alternative to EtBr
Suitable to blue or UV light
[RQ2110] ExcelRT™ 2X One-Step RT-qPCR Kit (TaqMan, ROX)
High yield
Reverse transcription at wide temperature range (42-60°C)
High specificity
Suitable for fast cycle program
With ROX reference dye
