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silantes/13C High Performance OD2 Media solution for E.coli/litre(s)/110201402

价格
¥11600.00
货号:110201402
浏览量:127
品牌:silantes
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商品描述
  • Description

Product description: E. coli CDN media OD2,13C-labelled

Content:

- 1 x Rich growth E.coli media, isotopic labelling: 13C, amount: 1 L, this product is in a ready-to-use sterilesolution,isotopic enrichment > 98 %

Silantes OD2 media are rich growth media for a high-performance expression of stable isotope labelled proteins. The media are formulated for a > OD2cell density (optical density measured at 600nm).

The media are made from bacterial hydrolysate and contain primarily amino acids, some low molecular weight oligopeptides and almost no carbohydrates.The bacterial strain used is a chemolithoautotrophic organism which grows on inorganic substrates that are isotopically labelled.

Note: Silantes media are natural products. A slightly different color (e.g. amongst different batches) is absolutely normal for natural products.

silantes蛋白质是细胞内所有活动的效应物。某些蛋白质的表达在细胞刺激或应激时或细胞分化或进入疾病状态时发生变化。蛋白质表达水平的测量以及其翻译后修饰的表征可提供有关其细胞状态的信息。定量蛋白质组学的目标是评估正在比较的两个细胞样品之间蛋白质的相对表达。质谱(MS)用于定量和鉴定分子。 FOR蛋白的准确定量,在细胞培养物中的氨基酸的稳定同位素标记(SILAC)是一种多功能的工具。 为此,需要在同位素标记方面与样品分子不同的参比分子(2 H,13 C,15 N)。Silantes提供了SILAC(在细胞培养中用氨基酸对同位素进行稳定同位素标记)和SILAM(在哺乳动物中对同位素进行稳定同位素标记)来生产这些参考分子的方法