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silantes/13C 15N大肠杆菌生态培养基粉/10克/117604300

价格
¥8000.00
货号:117604300
浏览量:97
品牌:silantes
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商品描述
  • Description

Product description: ECO powder media, 13C15N-labelled for E. coli

Content:

- 1 x ECO powder media, isotopic labelling: 13C15N, amount: 10 g, this product is in powder form, isotopicenrichment > 98 atom %

Silantes ECO media are a budget solution for the expression of stable isotope labelled proteins. They are made up from labelled salts and labelledcarbohydrates and supplemented with SILEX powder media.

The media are made from bacterial hydrolysate and contain primarily amino acids, some low molecular weight oligopeptides and almost nocarbohydrates.The bacterial strain used is a chemolithoautotrophic organism which grows on inorganic substrates that are isotopically labelled.

Note: Silantes media are natural products. A slightly different color (e.g. amongst different batches) is absolutely normal for natural products.

silantes蛋白质是细胞内所有活动的效应物。某些蛋白质的表达在细胞刺激或应激时或细胞分化或进入疾病状态时发生变化。蛋白质表达水平的测量以及其翻译后修饰的表征可提供有关其细胞状态的信息。定量蛋白质组学的目标是评估正在比较的两个细胞样品之间蛋白质的相对表达。质谱(MS)用于定量和鉴定分子。 FOR蛋白的准确定量,在细胞培养物中的氨基酸的稳定同位素标记(SILAC)是一种多功能的工具。 为此,需要在同位素标记方面与样品分子不同的参比分子(2 H,13 C,15 N)。Silantes提供了SILAC(在细胞培养中用氨基酸对同位素进行稳定同位素标记)和SILAM(在哺乳动物中对同位素进行稳定同位素标记)来生产这些参考分子的方法