To simplify the use of minimal medium, Scarab Genomics offers a two component kit consisting of 10X Modified Korz Medium and a separate 50X Magnesium Sulfate solution. The concentrated minimal medium and associated Magnesium Sulfate solution are diluted to create a 1X medium. A carbon source must then be added to support cell growth. We would recommend the equivalent of 0.2% glucose. The diluted 1X medium (with carbon source and appropriate antibiotics) is used for expression optimization in shake flasks. The same medium can also serves as the “batch” phase medium in fed-batch fermentations. Typically, the level of the carbon source would be adjusted to a higher level than that used in the shake flask e.g. when using glucose, the glucose level would be increased to 0.5%. Scarab’s Clean Genome® strains were specifically designed for the production of biotherapeutic protein and DNA. The “cleanest” medium to use for biotherapeutic production is a chemically defined, minimal medium. Accordingly, Modified Korz Minimal Medium has been extensively tested with the Scarab Clean Genome® Strains to verify its ability to support cell growth and the production of recombinant protein. Korz minimal medium was originally designed for high density fed-batch fermentation of E. coli (Korz et al. 1995). The medium consists of phosphate buffer, magnesium, ferric citrate, trace elements. The user needs to supply the carbon source. The same base medium used for optimizing expression in shake flasks can also be used for fed-batch fermentation, thereby providing continuity between the two processes. In fed-batch fermentations, the same medium is simply supplemented with higher carbon source content.

Kit Components

White Glove IS Detection Kit MDS™42 Chemically Competent Cell Kit MDS™42 ΔrecA Chemically Competent Cell Kit MDS™42 ΔrecA Blue Chemically Competent Cell Kit MDS™42 Combination Package Chemically Competent Cell Kit ScarabXpress® T7 lac Chemically Competent Cell Kit MDS™42 Electrocompetent Cell Kit MDS™42 ΔrecA Electrocompetent Cell Kit MDS™42 ΔrecA Blue Electrocompetent Cell Kit MDS™42 ΔrecA trfA Electrocompetent Cell Kit MDS™42 ΔrecA trfA Blue Electrocompetent Cell Kit MDS™42 Combination Package Electrocompetent Cell Kit

Product Manuals 10X Modified Korz Medium Kit Papers

1. Korz DJ, Rinas U, Hellmuth K, Sanders EA, Deckwer WD. J Biotechnol. (1995) Feb 21;39(1):59-65. Simple fed-batch technique for high cell density cultivation of Escherichia coli.
2. Pósfai G, et al., (2006) Emergent properties of reduced-genome Escherichia coli. Science 312:1044-6.

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