To simplify the use of minimal medium for fed-batch fermentations, Scarab Genomics offers medium for both the batch phase and feeding phase of fed-batch fermentations. The Korz Feed Stock supplies glucose, magnesium, iron, and trace elements for the feeding stage of Modified Korz Medium fed-batch fermentations. Scarab’s Clean Genome® strains were specifically designed for the production of biotherapeutic protein and DNA. The “cleanest” medium to use for biotherapeutic production is a chemically defined, minimal medium. Accordingly, Modified Korz Minimal Medium has been extensively tested with the Scarab Clean Genome® Strains to verify its ability to support cell growth and the production of recombinant protein. Korz minimal medium is designed for high density fed-batch fermentation of E. coli (Korz et al. 1995, Sharma et al. 2007). The medium consists of phosphate buffer, magnesium, ferric citrate, trace elements, and uses glucose as the carbon source. The same base medium used for optimizing expression in shake flasks can also be used for fed-batch fermentation, thereby providing continuity between the two processes.

Kit Components Korz Feed Stock, 1000 ml Quality Control The media is confirmed for growth of Clean Genome® Strains using liquid culture and confirmed aseptic by no growth in liquid media and agar plates. Storage Conditions For long term storage, store the Korz Feed Stock at 4-12°C and protected from light. For short term storage the Feed Stock may be stored on the bench top at room temp for several days.

10X Modified Korz Medium Kit, Sterile White Glove IS Detection Kit MDS™42 Chemically Competent Cell Kit MDS™42 ΔrecA Chemically Competent Cell Kit MDS™42 ΔrecA Blue Chemically Competent Cell Kit MDS™42 Combination Package Chemically Competent Cell Kit ScarabXpress® T7 lac Chemically Competent Cell Kit MDS™42 Electrocompetent Cell Kit MDS™42 ΔrecA Electrocompetent Cell Kit MDS™42 ΔrecA Blue Electrocompetent Cell Kit MDS™42 ΔrecA trfA Electrocompetent Cell Kit MDS™42 ΔrecA trfA Blue Electrocompetent Cell Kit MDS™42 Combination Package Electrocompetent Cell Kit

Product Manuals Korz Feed Stock, Sterile Papers

1. Korz DJ, Rinas U, Hellmuth K, Sanders EA, Deckwer WD. J Biotechnol. 1995 Feb 21;39(1):59-65. Simple fed-batch technique for high cell density cultivation of Escherichia coli.
2. Sharma S, Campbell JW, Frisch D, Blattner FR, Harcum SW. Biotechnol Bioeng. 2007 Dec 1;98(5):1056-70. Expression of Two Recombinant Chloramphenicol Acetyltransferase Variants in Highly Reduced Genome Escherichia coli Strains.
3. Akesson M, Karlsson EN, Hagander P, Axelsson JP, Tocaj A. Biotechnol Bioeng. 1999 Sep 5;64(5):590-8. On-line detection of acetate formation in Escherichia coli cultures using dissolved oxygen responses to feed transients.
4. DeLisa MP, Li J, Rao G, Weigand WA, Bentley WE. Biotechnol Bioeng. 1999 Oct 5;65(1):54-64. Monitoring GFP-operon fusion protein expression during high cell density cultivation of Escherichia coli using an on-line optical sensor.
5. Pósfai G, et al., (2006) Emergent properties of reduced-genome Escherichia coli. Science 312:1044-6.

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