Scarab’s Clean Genome® strains were specifically designed for the production of biotherapeutic protein and DNA. The “cleanest” medium to use for biotherapeutic production is a chemically defined, minimal medium. Accordingly, Ultra HD Minimal Feed Media formulation resulted from Scarab Genomics’ systematic determination of the metabolic needs of the Clean Genome® E. coli strains in fed batch fermentation for a variety of protein and DNA targets. Ultra HD Minimal Feed Media together with the optimized metabolism of the Clean Genome® strains enable ultra high densities up to 300 OD600 resulting in proteins yields of >40 g/L of test protein and 700 – 1000 mg/L plasmid in 10L scale fermentations without The medium consists of phosphate buffer, magnesium, ferric citrate, trace elements, and uses glucose as the carbon source. The same base medium used for the batch phase of fed-batch fermentation can be used in optimizing expression in shake flasks, thereby providing continuity between the two processes. A separate Feed Medium (Cat. No. S300-F-1L) supplies glucose, magnesium, iron, and trace elements for the feeding stage of fed-batch fermentation.

Kit Components Ultra HD Minimal Feed Media with 62.8% Glucose, 1X, Sterile Quality Control The media is confirmed for growth of Clean Genome® Strains using liquid culture and confirmed aseptic by no growth in liquid media and agar plates. Storage Conditions Store at +20-25ºC and protected from light.

Ultra HD Minimal Batch Media with 1% Glucose, 1X, Sterile White Glove IS Detection Kit MDS™42 Chemically Competent Cell Kit MDS™42 ΔrecA Chemically Competent Cell Kit MDS™42 ΔrecA Blue Chemically Competent Cell Kit MDS™42 Combination Package Chemically Competent Cell Kit ScarabXpress® T7 lac Chemically Competent Cell Kit MDS™42 Electrocompetent Cell Kit MDS™42 ΔrecA Electrocompetent Cell Kit MDS™42 ΔrecA Blue Electrocompetent Cell Kit MDS™42 ΔrecA trfA Electrocompetent Cell Kit MDS™42 ΔrecA trfA Blue Electrocompetent Cell Kit MDS™42 Combination Package Electrocompetent Cell Kit

Product Manuals Ultra HD Minimal Feed Media with 62.8% Glucose, 1X, Sterile Papers

1. Pósfai G, et al., (2006) Emergent properties of reduced-genome Escherichia coli. Science 312:1044-6.

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