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蚂蚁淘在线 / 品牌中心 / PromoCell / 促增殖细胞/人M2巨噬细胞（M-CSF）单核细胞衍生的

促增殖细胞/人M2巨噬细胞（M-CSF）单核细胞衍生的

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面议
货号：C-12917
浏览量：127
品牌：PromoCell
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商品描述

PromoCell’s crypreserved human monocyte-derived macrophages (hMDM) are convenient and easy-to-handle. The thawed cells plate into all tissue culture vessel formats and can be maintained as adherent, biologically functional cultures for several weeks. Optionally, user-customizable activation of the cells can be performed.

Cryopreserved human macrophages are produced from monocytes in PromoCell’s well-proven M2 Macrophage Generation Media DXF and are available as non-activated, fully qualified M2- (M-CSF) polarized cells. After 9-10 days of differentiation, the M2 macrophages are cryopreserved using PromoCells’s proprietary, defined and animal-free freezing medium, Cryo-SFM. Each cryo vial contains more than 1.5 million or 5 million viable cells after thawing, respectively.

NEW: Our M2 Macrophages are now also available from HLA-typed donors.

Recommended plating density200,000 cells per cm2
Tested MarkersCD163 positive, CD68 positive
CryoMacrophages an Overview
Figure 1. Culture of PromoCell"s cryopreserved human macrophages. The macrophages can be directly used for experiments after thawing and plating. Optional: activation of polarized M1-/M2 macrophages 24h before assay.
CryoMacrophages an Overview
Figure 1. Culture of PromoCell"s cryopreserved human macrophages. The macrophages can be directly used for experiments after thawing and plating. Optional: activation of polarized M1-/M2 macrophages 24h before assay.
CryoMacrophages-FACS
Figure 2. Flow cytometric analysis of PromoCell cryopreserved Human M1 and M2 Macrophages. M1 macrophages exhibit a CD68+ (99,74%) and CD80+ (83,33%) marker expression profile, typical for M1 macrophages (upper row). M2 macrophages exhibit a CD68+ (99,72%) and CD163+ (94,91%) marker expression profile, typical for M2 macrophages (lower row).
CryoMacrophages-FACS
Figure 2. Flow cytometric analysis of PromoCell cryopreserved Human M1 and M2 Macrophages. M1 macrophages exhibit a CD68+ (99,74%) and CD80+ (83,33%) marker expression profile, typical for M1 macrophages (upper row). M2 macrophages exhibit a CD68+ (99,72%) and CD163+ (94,91%) marker expression profile, typical for M2 macrophages (lower row).
国PromoCell是细胞及细胞培养产品的主要制造商，公司主要致力于提供各种正常人类原代细胞、干细胞和血细胞制品、细胞培养的无血清无蛋白培养基、各类细胞培养液、细胞生长因子及无血清细胞冻存液等。此外， PromoCell还提供大量成熟的细胞生物学科研产品，例如细胞分析试剂盒、细胞转染试剂、荧光染色剂、抗体、ELISA和细胞因子，Promocell还提供各类抗体、分子生物学相关试剂、检测试剂盒。Promocell所研发的各种相关产品，几乎覆盖细胞研究的所有领域，已被全球著名制药企业和研究院校的科研人员广泛使用。现部分产品现货供应，数量不多，先到先得。