PMA(propidiummonoazide)dyeisaDNAmodifierinventedbyscientistsatBiotium.Itisaphoto-reactiveDNA-bindingdyethatisusefulfordeterminationofmicrobialviABIlitybyqPCR.

PMA(propidiummonoazide)dyeisaDNAmodifierinventedbyscientistsatBiotium.Itisaphoto-reactivedyethatbindstodsDNAwithhighaffinity.UponphotolysiswithvisIBLelight,PMAdyecovalentlyattachesitselftodsDNA.ThePMA-modifieddsDNAcannotbeamplifiedbyPCR.Thedyeisdesignedtobecellmembrane-impermeable.Thus,inapopulationofliveanddeadcells,onlydeadcellsaresusceptibletoDNAmodificationduetocompromisedcellmembranes.ThisuniquefeatureofPMAdyemakesithighlyusefulinselectivedetectionoflivebacteriabyqPCR.Abacterialsamplecanbefirstpre-treatedwithPMAunderlighttoinactivatedsDNAfromdeadcells.DNAisthenextractedandanalyzedbyPCR.SinceBiotiumfirstdevelopedPMAdye,therehavebeenhundredsof publicationsontheuseofthedyeinpathogenicbacterialdetectionrelatedtofoodandwatersafety,medicaldiagnosisandbiodefense;downloadapartial PMAReferenceList.PMAisalsoavailableinlyophilizedformat,catalogno.40013.

Note:ScientistsatBiotiumhaverecentlyinventedanewandimproveddyeforviabilityPCR,calledPMAxx™(40069).PMAxx™functionsinamannerverysimilartoPMA,buthasmuchgreateractivityandabilitytodistinguishbetweenliveanddeadbacteria.PMAxx™canbeusedasareplacementforPMAinanybacterialviability-PCRassay.WerecommendthatcustomersinterestedinusingPMAtryPMAxx™instead,forimproveddetectionofviablebacteria.

ForviabilityPCRofgram-negativebacteriawehighlyrecommendusingournewPMAEnhancer(31038)inconjunctionwithPMA.PMAEnhancerforGramNegativeBacteriawasdesignedtoimprovePMA-mediateddiscriminationbetweenliveanddeadgram-negativebacteria.Whenasequencefromagram-negativebacteriaisamplifiedbyPCR,samplespre-treatedwithEnhancershowadecreaseinthesignalfromdeadcells,withnochangeinthesignalfromlivecells.

Biotiumalsoprovidesstrain-specificPMAReal-TimePCRBacterialViabilitykitswithvalidatedprimersfor7selectedpathogens:M.tuberculosis,S.aureus,MRSA,Salmonella,E.coli,E.coliO157:H7andListeria.Thesekitsprovideeverythingthatyouneedfortheselectivedetectionofyourfavoritespeciesoflivebacteriabyreal-timePCR.Don’tseeyourfavoritestrain?Letusknowattechsupport@biotium.com.

Real-TimePCRBacterialViabilityKits

ForphotoactivationofPMAxx™,PMAorEMAdye,werecommendtheuseofBiotium’sPMA-Lite™LEDPhotolysisDevice(E90002),whichisdesignedtoconductphotolysisundercontrolledconditions.

• Molecularweight:511
• Abs=464nm(beforephotolysis)
• Abs/Em=510/610nm(followingphotolysisandcovalentattachmenttoDNA/RNA)
• Darkredliquid
• Storeat-20°Candprotectfromlightatalltimes

Biotium 5(6)-羧基-X-罗丹明-N-羟基琥珀酰亚胺酯 Biotium 异硫氰酸荧光素酯 Biotium 10-壬基吖啶橙溴化物 Biotium 4-甲基伞形酮磷酸二钠盐三水 Biotium 河豚毒素 Biotium 2,2'-联氮双(3-乙基苯并噻唑啉-6-磺酸)二铵盐 Biotium N,N-二甲基甲酰胺 Biotium N-生物素酰基-L-赖氨酸酰肼 Biotium 5-溴-6-氯-3-吲哚基-beta-D-半乳糖苷 Biotium N,N,N',N'-四(2-吡啶甲基)乙二胺 Biotium 腔肠素-H Biotium Α-金环蛇毒 Biotium 5-溴-4-氯-3-吲哚半乳糖苷 Biotium BIOTIN-(AC5)2-OSU Biotium 4',6-二脒基-2-苯基吲哚二盐酸盐 Biotium 4-甲基伞形酮磷酸酯 Biotium 90% (TLC) Biotium 96314-98-6 SULFORHODAMINE G 5873-16-5 5(6)-CARBOXY-2',7'-DICHLOROFLUORESCEIN DIACETATE 127770-45-0 HYDROETHIDINE 38483-26-0 SULFORHODAMINE 101 60311-02-6 L-N6-(1-IMINOETHYL)LYSINE DIHYDROCHLORIDE 150403-89-7