Fireflyluciferaseiswidelyusedasareporterforstudyinggeneregulationandfunction,andforpharmaceuticalscreening.Biotium’sSteady-Luc™HTSassaysystem isahomogeneousfireflyluciferasereportergeneassaykitwithahalf-lifeofabout3hoursforthequantificationoffireflyluciferaseexpressioninmammaliancells,allowingbatchprocessinginmicroplates.

Fireflyluciferaseiswidelyusedasareporterforstudyinggeneregulationandfunction,andforpharmaceuticalscreening.Itisaverysensitivegeneticreporterduetothelackofendogenousactivityinmammaliancellsortissues.However,thelightproductionresultingfromthereactionleadstoformationofsuicidaladenyl-oxyluciferinattheenzymesurface.Itresultsinveryshorthalf-lifeofthelightemissionwithaflash-typekinetics.Becauseofthis,flash-typeluminescencemustbedetectedimmediatelyafterinitiatingthereaction,whichisnotcompatIBLewithbatchanalysisofsamples.

Biotium’sSteady-Luc™HTSassaysystemisaproprietarymixtureofsubstancesthatmodifytheenzymaticreactiontoproducealonglastingsignal(steadyglow)bypreventingtheformationofadenyl-oxyluciferinattheenzymesurface.Itisahomogeneoushighsensitivityfireflyluciferasereportergeneassaykitwithahalf-lifeofabout3hoursforthequantificationoffireflyluciferaseexpressioninmammaliancells,allowingbatchprocessinginmicroplates.

Glow-typeluciferaseassayslikeSteady-Luc™havelowerluminescencesignalcomparedtoflash-typeassays.Thesensitivityandlimitofdetectionoftheassaywilldependonluciferaseexpressionlevelsinyourexperimentalsystemaswellasluminometersensitivity.

BiotiumalsooffersSteady-Luc™FireflyHTSAssayKit(Lyophilized)(catalogno.30028L),anewpackagingformatthatincludeslyophilizedassaybufferforconvenientroomtemperatureshippingandstorageat-20°C.

KitComponents:

• D-Luciferin
• Steady-LucFireflyAssayBuffer

Features:

• Simple:singlestephomogeneousassay.
• Glowkinetics:luminescencehalflifeofabout3hours.
• Economic:saveupto45%comparedtootherbrands.

MoreLuciferaseKits

Catalognumber	Product	Description
30085	FireflyLuciferaseAssayKit2.0	Flash-typefireflyluciferaseassaykit.
30075	FireflyLuciferaseAssayKit(Lyophilized)	Fireflyluciferaseassaykitwithlyophilizedassaybufferforeconomicalroomtemperatureshippingandconvenientstorageat-20°C.
99923	5XFireflyLysisBuffer	ExtralysisbufferforusewithFireflyLuciferaseAssayKits,onlyrequiredifusingwellsizeslargerthan24-well.
30028	Steady-Luc™FireflyHTSAssayKit	Glow-typefireflyluciferaseassaywithsignalhalf-lifeofabout3hoursforhigh-throughputscreeninginmulti-wellplates.
30028L	Steady-LucFireflyHTSAssayKit(Lyophilized)	Steady-Luckitwithlyophilizedassaybufferforeconomicalroomtemperatureshippingandconvenientstorageat-20°C.
30082	RenillaLuciferaseAssayKit2.0	Flash-typeRenillaluciferaseassaykit.
99912	5XPassiveLysisBuffer	ExtralysisbufferforusewithRenillaLuciferaseAssayKits,onlyrequiredifusingwellsizeslargerthan24-well.
30081	FireflyandRenillaLuciferaseSingleTubeAssayKit	AssaykitformeasuringfireflyandRenillaactivitiessequentiallyinthesamesampleinasingletube.
99821-100mL	1XPassiveLysisBuffer2.0	ExtralysisbufferforusewithFirefly&RenillaSingleTubeAssayKits,onlyrequiredifusingwellsizeslargerthan24-well.
30020	ATP-Glo™BioluminometricCellViABIlityAssayKit	Flash-typeluminescence-basedassayformeasuringcellviabilityorATPconcentration.
22003	MiniCellScrapers	Mini-cellscrapers,usefulforlysingcellsin96-well,48-well,or24-wellplates.

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