ATP-Glo™BioluminometricCellViABIlityAssayoffersahighlysensitiveassayforquantifyingATP.ThehomogeneousassayprocedureinvolvesasingleadditionofATP-Glo™DetectionCocktaildirectlytocellsculturedinaserum-supplementedmedium.

ATP-Glo™BioluminometricCellViabilityAssayoffersahighlysensitiveassayforquantifyingATP.ThehomogeneousassayprocedureinvolvesasingleadditionofATP-Glo™DetectionCocktaildirectlytocellsculturedinaserum-supplementedmedium.Itisnotnecessarytoremovecellculturemediumorwashcellsbeforeaddingthereagent.

BecauseATPisanindicatorofmetabolicallyactivecells,thenumberofviablecellscanbeassessedbasedontheamountofATPavailable.ThisATPdetectionkittakesadvantageoffireflyluciferase’suseofATPtooxidizeD-LuciferinandtheresultingproductionoflightinordertoassesstheamountofATPavailable.

TheATP-Glo™kitcanbeusedtodetectaslittleasasinglecellor0.01picomoleofATP.Thesignalproducedislinearwithin6ordersofmagnitude.ByrelatingtheamountofATPtothenumberofviablecells,theassayhaswideapplications,rangingfromthedeterminationofviablecellnumberstocellproliferationtocellcytotoxicity.

Pleasenote:ATP-Glo™isaflash-typeluminescenceassay.Theluminescencesignalgeneratedisstableforupto1minute.Thisassayisdesignedforindividualsampledetectionbyusingaluminometerinasinglesampleformatoraluminometerwithaninjectorin96-wellplateformat.

KitComponents:

• D-Luciferin
• Fireflyluciferase
• ATP-Glo™assaybuffer
• 2mMATPstandard

Features:

• HighSensitivityandExtendedLinearity:Detectfromasinglecelltoseveralmillioncellswithexcellentlinearity
• Simple:Asingle-stephomogenousassay
• Versatile:DetectATPamountorquantifynumberoflivecells

MoreLuciferaseKits

Catalognumber	Product	Description
30085	FireflyLuciferaseAssayKit2.0	Flash-typefireflyluciferaseassaykit.
30075	FireflyLuciferaseAssayKit(Lyophilized)	Fireflyluciferaseassaykitwithlyophilizedassaybufferforeconomicalroomtemperatureshippingandconvenientstorageat-20°C.
99923	5XFireflyLysisBuffer	ExtralysisbufferforusewithFireflyLuciferaseAssayKits,onlyrequiredifusingwellsizeslargerthan24-well.
30028	Steady-Luc™FireflyHTSAssayKit	Glow-typefireflyluciferaseassaywithsignalhalf-lifeofabout3hoursforhigh-throughputscreeninginmulti-wellplates.
30028L	Steady-LucFireflyHTSAssayKit(Lyophilized)	Steady-Luckitwithlyophilizedassaybufferforeconomicalroomtemperatureshippingandconvenientstorageat-20°C.
30082	RenillaLuciferaseAssayKit2.0	Flash-typeRenillaluciferaseassaykit.
99912	5XPassiveLysisBuffer	ExtralysisbufferforusewithRenillaLuciferaseAssayKits,onlyrequiredifusingwellsizeslargerthan24-well.
30081	FireflyandRenillaLuciferaseSingleTubeAssayKit	AssaykitformeasuringfireflyandRenillaactivitiessequentiallyinthesamesampleinasingletube.
99821-100mL	1XPassiveLysisBuffer2.0	ExtralysisbufferforusewithFirefly&RenillaSingleTubeAssayKits,onlyrequiredifusingwellsizeslargerthan24-well.
30020	ATP-Glo™BioluminometricCellViabilityAssayKit	Flash-typeluminescence-basedassayformeasuringcellviabilityorATPconcentration.
22003	MiniCellScrapers	Mini-cellscrapers,usefulforlysingcellsin96-well,48-well,or24-wellplates.

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